Chromatofocusing

Chromatofocusing is a chromatography technique that separates proteins according to differences in their isoelectric point (pI). The pI of each protein is the pH at which the protein has zero surface charge. Proteins with different pI can be separated by being passed through a chromatofocusing column (packed with a specific medium) while a pH gradient is generated on the column by specifically designed and matched amphoteric buffers. Proteins elute in order of their isoelectric points. To achieve consistently high resolution, generation of the linear pH gradient requires an even buffering capacity over the entire pH range used for a separation. Hence the need for specifically designed buffers (Polybuffer 74 or Polybuffer 96) and media substituted with charged, buffering amines (Mono P).

Chromatofocusing Products

Mono P™ columns

Lab Pack

Polybuffer

Chromatofocusing

Handbooks

Ion Exchange Chromatography & Chromatofocusing: Principles & Methods pdf

Protein Purification Handbook pdf