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Protein Purification
Overview
Applications
Tagged protein purification
Antibody purification
Membrane protein purification
General protein purification
Chromatography techniques
Affinity
Chromatofocusing
Gel Filtration & Desalting
Hydrophobic Interaction
Ion Exchange
Reversed Phase
Chromatography columns
Prepacked columns
Empty columns
C columns
HiScale columns
HR columns
PD-10 columns
SR columns
Tricorn columns
XK and K 9 columns
Customized columns
Chromatography systems
Filtration
Education center

Empty columns

Our chromatography columns offers quality, versatility, precision, and engineering excellence. Combined with an extensive range of media, they provide outstanding performance.

HiScale™ columns are designed to withstand pressures of up to 20 bar (290 psi) and high flow rates, making them compatible with modern BioProcess chromatography media such as MabSelect™ and Capto™.

XK columns are specified to run all media including Superdex™ prep grade and Sepharose™ High Performance media.

C columns are economical and well-proven.

SR columns are solvent-resistant and used for chromatography involving organic eluents.

Tricorn™ empty, high-performance glass columns are ideal for high-performance media such as Sepharose™ High Performance, Superdex™ prep grade, and SOURCE™. When working with capture media such as Capto™, MabSelect™ or Sepharose™ Fast Flow, Tricorn™ Coarse Filter Kit (see Tricorn Empty High-Performance Columns) is recommended to reduce the risk of clogging.

Empty PD-10 columns are economical, plastic columns for single-use applications using gravity flow. These easy to use columns are suitable for a range of applications using Sephadex and Sepharose media.

HR columns for large scale applications using high performance media

Selection Guide Empty columns

• = Compatible
- = Not Compatible
** = HR columns are mainly intended for high resolution media
*** = SR columns are intended for use when high solvent resistance is needed

Lab packs
________________________________________________________________________________________________
Affinity
________________________________________________________________________________________________
Sepharose/4B/CL-4B/6B
**
***
Sepharose FF
**
***
Sepharose HP
-
***
-
MabSelect
-
**
-


Gel Filtration
________________________________________________________________________________________________
Sephacryl
-
***
-
Sephadex
**
***
Sepharose FF
***
Superdex prep grade
-
***
-
Superose
-
***
-


Hydrophobic interaction
________________________________________________________________________________________________
Sepharose FF
**
***
Sepharose HP
-
***
-
SOURCE 15
-
-
-
-
SOURCE 30
-
-
-


Ion Exchange
________________________________________________________________________________________________
Capto
-
-
***
Macrocap
**
-
***
Sepharose FF
**
***
Sepharose HP
-
***
-
Sepharose XL
**
***
SOURCE 15
-
-
-
-
-
SOURCE 30
-
-
-
-


Reversed phase
_______________________________________________________________________________________________
SOURCE 15
-
-
-
-
-
SOURCE 30
-
-
-
-



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