Reversed Phase Chromatography

Reversed Phase Chromatography (RPC) separates molecules according to differences in their hydrophobicity. RPC has become increasingly important for high-resolution separation and analysis of proteins, peptides and nucleic acids. The technique is ideal for applications such as peptide mapping or purity checking and is often used for final polishing of oligonucleotides and peptides. Separations can be performed using isocratic elution; jpwever, most frequently, gradient elution is used in order to minimize the run time. RPC can also be used to separate molecules of significantly differetd hydrophobicity using step elution.

Reversed Phase Products

Application area

High-resolution analysis and purification of peptides, proteins, and oligonucleotides, especially when high pH is needed, and for use in LC-MS applications

Unique selectivity. Preparative purification of proteins, peptides, and oligonucleotides, especially at high pH

Ideal for very high resolution analysis and preparative micropurificatio

Reversed Phase Chromatography

Handbooks

Hydrophobic Interaction and Reversed Phase Chromatography pdf

Protein Purification Handbook pdf