Storm – the economical standard for phosphorimaging and red/blue fluorescence

Storm is the economical laboratory standard for quantitative phosphorimaging, ECL™ Plus Westerns, ECL Plex™ multiplex Westerns, and red and blue excited fluorescence in gels and blots. The Storm 825 model provides phosphorimaging only, with up to 50 µm resolution for gel and blot imaging. The Storm 845 model adds 450 nm excitation for ECL Plus and nucleic acid dyes, and the Storm 865 model adds multiplex 450/635 nm excitation for ECL Plex multiplex Westerns. Storm - Features and benefits Imaging of radioisotopes by storage phosphor 3H, 14C, 32P, 33P, 35S, 125I Up to 100 times faster and more quantitative than film

Scan area of 35 x 43 cm

• Suitable for multiple gels and blots as well as DNA sequencing gels and macroarrays

Red and blue fluorescence

• Easy migration from radioactive to fluorescent imaging
• Images ECL Plus chemifluorescent Westerns (Storm 845 and 865 only)
• Images ECL Plex Westerns to quantitate two proteins (Storm 865 only)

Average laser lifetimes of 60 000 h

• Low cost of ownership

Includes ImageQuant TL software

• Seamless transfer to quantitative image analysis designed for Storm.
  
  

Technical specification

Detection modes	Phosphorimaging, chemifluorescence (Storm 845 and 865 only) multifluorescence (Storm 865 only)
Radioisotopes	3H, 14C, 32P, 33P, 35S sources
Dynamic range	Five orders of magnitude
Bit depth	16-bit
Max scanning area	35 x 43 cm
Pixel sizes	50, 100, 200 µm
Dimensions	760 (W) x 760 (D) x 380 (H) mm
Line frequency	50/60 Hz
Temperature	15 - 30 °C
Humidity	30 - 70 % (no condensation)
Supply voltage	115 - 230 VAC
Power consumption	Less than 0.5 kVA

Excitation wavelengths

Light	Laser type	Estimated average lifetime	Wavelength
Red1	LED	60 000 h	635 nm
Blue2	LED	60 000 h	450 nm

¹Storm 865 only; ²Storm 845/865 only

Imaging Application

Ratings are based on overall system performance including model-specific features, versatility, and sensitivity (limit of detection).

++++ Superior performance
+++ High performance
++ Good performance
+ Acceptable performance
- Not compatible

Imaging Applications I	Storm 865	Storm 845	Storm 825
Storage Phosphor
32P, 125I, 14C, 35S, 33P, 3H	++++	++++	++++
Macroarray (radiolabeled)	+++	+++	+++
Fluorescence—Proteins
ECL Plex Fluors
Cy2	++++	-	-
Cy3	-	-	-
Cy5	++++	-	-
Protein Stains
SYPRO Ruby	+++	+++
Chemifluoresence (enzyme-catalyzed)
ECL Plus Western blotting	++++	++++	-
Multipurpose fluorescence
Cy2	++++	+++	-
Cy5	++++	-	-

Storm™

Datafile

Storm Imager pdf