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Larger scale sample prep and protein purification

GST-tagged Protein Capture selection guide

Anti-GST AntibodyUnconjugated antibody with affinity for the GST tag
E. coli BL21Bacterial strain for the expression of GST fusion protein
Factor XaSite-specific cleavage of the GST tag from fusion proteins expressed using pGEX-X vectors
GST 96- Well detection ModuleReagents and plates with precoated wells for the detection of GST-tagged proteins
GST Buffer KitBuffer concentrates for binding, washing, and elution of up to 20 mg of GST-tagged protein
GST Bulk KitBulk Glutathine Sepharose 4B, empty gravity columns, IPTG, and GST Buffer Kit for purification by column chromatography or batch methods
GST GraviTrapFor fast and simple manual purification of GST-tagged proteins by gravity flow
GST MultiTrap™ 4BPurification of GST-tagged proteins in larger series or automation in 96-well format
GST MultiTrap FFPurification of GST-tagged proteins in larger series or automation in 96-well format
GST SpinTrap™Purification of up to 500 µg of GST-tagged protein using a microcentrifuge
GST Vector primers for sequencingSequencing primers for the pGEX vectors
GSTPrep™ FF 16/10 columnSimple use with a syringe or chromatography system such as ÄKTA™
GSTrap™ 4B columnsSimple use with a syringe or chromatography system such as ÄKTA
GSTrap FF columnsSimple use with a syringe or chromatography system such as ÄKTA
GSTrap HP columnsSimple use with a syringe or chromatography system such as ÄKTA
M13K07 Helper PhageM13 phage for the GST expression system
pGEX vectorsExpression vectors for GST fusion proteins
PreScission™ ProteaseSite-specific cleavage of the GST tag from expressed fusion proteins
RediPack GST Purification ModulePurification of GST-tagged proteins in gravity-flow format
ThrombinSite-specific cleavage of the GST tag from fusion proteins expressed using the pGEX-T vectors


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