Affinity chromatography

Affinity chromatography is a purification technique that typically offers purities >95% in one step. It makes use of a specific native or added property of the target molecule to isolate it from all contaminants in the sample. Binding sites of receptors and antibodies or active sites of enzymes are examples of very specific properties that can be used for affinity chromatography. Prosthetic groups like metal ions are also used, but then for group separations. Group separations can also be accomplished by using ligands with broader specificity, for example immobilized lectins for isolating and separating glycoproteins . When a ready-to-use affinity medium is not available, a medium can be designed for the purification of one or more target molecules by coupling a specific ligand onto a pre-activated chromatography matrix.

Affinity chromatography

Selection guide

Afffinity Chromatography columns and media pdf

Handbook

Affinity Chromatography: Principles and Methods pdf

Protein Purification Handbook pdf

Recombinant Protein Purification: Principles and Methods pdf