HiLoad™ columns

HiLoad columns are XK laboratory columns prepacked with high-performance media for convenient and reliable protein purification. Choose HiLoad columns when high resolution and high capacity are essential criteria at laboratory scale. Prepacked HiLoad columns are available in 120 ml and 320 ml formats for gel filtration and 20 ml and 53 ml formats for ion exchange and hydrophobic interaction chromatography at laboratory scale. Complete, easy-to-follow instructions are included with each pack of columns.

HiLoad™ columns

Gel Filtration

High-resolution purification of peptides, small proteins, and oligosaccharides with a molecular weight < M_r 10 000

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High-resolution purification of peptides, proteins, and oligosaccharides, M_r 3000-70 000

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High-resolution purification of antibodies and large proteins > M_r 10 000-600 000

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Hydrophobic Interaction

Purification of peptides and proteins with high hydrophobicity; high resolution prepacked medium, ideal for scale-up and intermediate purification of proteins after ion exchange chromatography

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Ion Exchange

Anion exchange (strong); high resolution and selectivity, purification of charged biomolecules, broad working pH range

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Cation exchange (strong); high resolution and selectivity, purification of charged biomolecules, broad working pH range

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