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HiTrap Heparin HP

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  • Price:
    CAD 243.00
  • Product code:
    17-0406-01
Complete Packsize:

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HiTrap Heparin HP

HiTrap Heparin HP columns are prepacked with Heparin Sepharose High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

  • Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
  • Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
  • Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.
  • Simple operation with a syringe, a pump or a chromatography system such as ÄKTA design

Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.

Heparin is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap Heparin HP columns equilibrated with 25 ml of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4°C to 30°C.

HiTrap Heparin HP

Flow Rate < 4 ml/min1)
Pressure Max. [Over the Packed Bed During Operation] 5 bar [0.5 MPa] (70 psi)
Storage Conditions 4 to 30°C, 20% Ethanol
Bed Dimensions 7 × 25 mm
Bed Volume 1 ml
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
Storage Conditions 4 to 30°C, 20% Ethanol
pH Stability Cleaning 5-10
pH Stability Working Range 5-10
Ligand Porcine heparin
Matrix Highly cross-linked agarose, 6%
Chemical Stability All commonly used buffers
Average Particle Size 34 μm
Ligand Concentration 10 mg heparin/ml medium
Column
Complete Packsize 1 ml
Material [Column Hardware] Polypropylene (PP)
Column i.d. 7 mm

HiTrap Heparin HP



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