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CNBr-Activated Sepharose 4B
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CNBr-Activated Sepharose 4B, 15 g
CNBr-activated Sepharose 4B is a pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose media, by the cyanogen bromide method, without an intermediate spacer arm.
- For immobilizing antibodies or other large proteins containing -NH2 groups by coupling them to the matrix
- Over 25 years proven track record for coupling ligands containing primary amino groups
- Multipoint attachment resulting in a chemically stable product
- The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out
CNBr-activated Sepharose 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.
CNBr-Activated Sepharose 4B, 15 g — Technical Specifications
|Average Particle Size||90 µm|
|Group To be Coupled||-NH2|
|Particle Size||45 μm-165 μm|
|Coupling Capacity||25 mg α-chymotrypsinogen/ml medium-60 mg α-chymotrypsinogen/ml medium|
|pH Stability Ligand Dependent||2-11|
|Pressure/Flow Specification||base matrix 70-140 cm/h, pressure drop cm H2O/bed height=15 cm, bed height 10 cm, 5 cm i.d.|
|Storage Conditions||2 to 8°C|
|Chemical Stability||Stable to all commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride. Stable in organic solvents, such as 50% dimethylformamide and 50% dioxane|