Default Organization

United Kingdom

Quick Order

Home

Products

Applications

Brands

Service & Support

About Us

Products

Product Catalog

Chromatography Systems and Software

Technology Insights

ÄKTAFPLC

Previous Next

1 (2)

Quote

Price:
On request
Product code:
18-1900-26

Overview Product Data Related Products Product Support

ÄKTAFPLC

ÄKTAFPLC is a preparative liquid chromatography system for fast and easy purification of proteins. The compact and flexible design with an expandable rack allows the use of longer chromatographic columns and automation kits.

Proven technology for protein purification.
Robust design of FPLC pump.
Expandable with automated functionality.

ÄKTAFPLC is a member of the ÄKTAdesign family, and a proven standard in protein purification. The FPLC system was developed in the early 80s, with features devised to help scientists overcome the difficulties of working with biological material. Building on this expericence, ÄKTAFPLC fully meets the demands of modern purification media by utilizing ÄKTAdesign and UNICORN to provide reliable results and system versatility, see UNICORN - for fast and efficient control of protein purification

The system pump, P-920 doubles the flow rate to 20 ml/min for fast separations using modern media. High-performance Monitor UPC-900 measures UV and temperature compensated conductivity and pH in a single instrument. (Flow cells for UV and conductivity are included, whilst the pH probe and flow cell are optional.) The integrated rack can be compacted to conserve bench space or expanded to hold large columns (with a handy clip design) or additional valves and components

ÄKTAFPLC

Complete Packsize	1 Piece
Application	Protein purification in lab scale using piston pump
Flow rate	0.05-20 ml/min
Flow Rate	0.05-20 ml/min
Operating Pressure Max.	5 MPa (725 psi)
Tubing i.d. [Flow Path]	0.5 mm
Single Wavelength Detection	Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm. Optional zink lamp with 214 nm filter.
Multiple Wavelength Detection	No
Flow Cell Path Length	5 mm (optional 2 mm)
pH Monitoring	2-12
Multiple Sample Injection	Optional, up to 8 samples
Autosampler Injection	Optional
Automatic Buffer Preparation	No
Buffer Selection	2 buffer inlets, option: 16 buffer inlets
Multi-Step Purification	No
Relative Humidity	20%-95% (non condensing)
pH Stability Long Term	1-13
pH Stability Short Term	1-14
Viscosity	max 10 cP
Width	440 mm
Height	480 mm
Depth	490 mm
Weight	39 kg
Voltage	100-120/220-240 VAC
Protection Class	IP 20
Compliance	The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended. For regulatory details please see declaration of conformity.

Storage Conditions	10 ROOM TEMPERATURE
Freight Conditions	ROOM TEMPERATURE
RoHS Compliance	Unknown
Certificate of Analysis	N
Min. Order Quantity	1

ÄKTAFPLC

Animation
HiTrap: Connecting to a chromatography system (ÄKTA) Close

Application note
Purification and chromatographic characterisation of an integral membrane protein Summary ÄKTAFPLC controlled by UNICORN™ software was used at cold room temperature for one-step purification and for characterisation of an integral membrane protein in the presence of non-ionic detergents. The target protein was a recombinant histidine-tagged cytochrome bo3 ubiquinol oxidase expressed in Escherichia coli. Detergent extracts of bacterial membranes were applied to HiTrap™ Chelating. Adsorbed proteins were eluted by an imidazol gradient in two well separated zones which both contained cytochrome bo3. The charge and size homogeneity of the purified protein was assessed under non-denaturing conditions, in the presence of non-ionic detergent by anion exchange chromatography on Mono Q™ HR 5/5 and by gel filtration on Superdex™ HR 10/30. A single sharp peak was observed for both fractions upon salt gradient elution of proteins adsorbed to the ion-exchanger, but minor peaks were detected for the low-imidazol fraction. Similarly, gel filtration gave essentially one single peak corresponding to monomeric protein. These results indicate that the preparations were homogenous in size and charge. Close

Application note

Purification and chromatographic characterisation of an integral membrane protein Summary

Brochure
Instrument Services for ÄKTA Systems Summary ÄKTA design is a platform of systems that gives you a competitive advantage in biomolecule purification and separation. Our service engineers are specifically trained to keep your ÄKTA system performing optimally, to save you time and maximize productivity, allowing you to rely on your results and concentrate on your research. Close
Protein purification - Applications that meet your needs Summary You must deliver quality results, pure protein targets and publish findings—in time and on budget. While it is complex work, purification need never to be an obstacle for you. Better still, it offers you opportunities to gain real competitive advantages. Here you’ll find proven solutions to many of today’s protein purification challenges. As well as an overview of the chromatography systems, services and quality assurances that we make available to support your science. With nearly 50 years of experience and a wealth of knowledge in purifying proteins, the Protein Separations specialists at GE Healthcare are ready to help you gain real competitive advantages. (40 pages) Close
Validation Services Summary GE Healthcare Validation Services supports efficient validation of your GE Healthcare equipment compliance status. Our documentation supports your equipment throughout its entire life cycle. When you choose GE Healthcare as your provider of validation support, you can be confident that your equipment is operating in a reliable, consistent, and GxP-compliant manner. Close
ÄKTA Protein purification by design Summary With the ability to purify virtually any biomolecule, ÄKTA design platform can handle the simplest and the toughest of challenges. It gives you speed, ease of use, and flexibility whatever your purification application or scale. The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to process development and manufacturing. Systems in the ÄKTA platform work with intelligent UNICORN software, which makes it simple to control every stage of your purification process. A broad range of prepacked columns, media, and filters provides more options for optimum results. Close
Äutomation Summary Are you purifying synthetic peptides or nucleic acids? Native or recombinant proteins? In today’s multi-disciplined research labs, protein purifi cation is a fundamental tool. The ÄKTAdesign™ platform provides versatility, high quality and built in knowledge to lighten the process of protein purifi cation. There are many ways it can add further functionality to make your everyday work even simpler and more rewarding. Standard ÄKTA™ confi gurations can easily be expanded into advanced setups to meet your individual purifi cation needs, increase reproducibility and reduce timeconsuming steps. Close

Flyer
Discontinuation of ÄKTApurifier and ÄKTAfplc systems - Moving to the future of protein purification Close

Software
UNICORN software strategy ÄKTAFPLC and Frac-920 or Frac-900 Summary The installation program downloads a software strategy for UNICORN 4.12 or higher, ÄKTAFPLC and Frac-920 or Frac-900. Close
UNICORN software strategy ÄKTAFPLC and Frac-950 Summary The installation program downloads a software strategy for UNICORN 4.12 or higher, ÄKTAFPLC and Frac-950. Double-click on the file and follow the instructions on the screen. After the installation the new strategy can then be chosen in UNICORN Manager by going to Administration - System Setup - Edit system and then choosing the new strategy. Close
UNICORN v.5.x Global Columns File Setup Summary Please read the installation instruction before installing the global columns file. Close

Software change description
UNICORN 5.2 vs 5.11 Summary This document describes the changes implemented in UNICORN™ 5.2 compared to the previous version 5.11. Close
UNICORN™ 5.31 vs 5.30 Software Change Description Summary This document describes the implemented changes and improvements in UNICORN 5.31 compared to the previous version 5.30. Close

Software information letter
UNICORN 5.31 Information Letter Summary This Information letter contains descriptions of different scenarios that may occur when UNICORN 5.31 is used. If applicable, actions to avoid or correct problems resulting from the described scenarios are suggested. This information is valid for UNICORN 5.31 software (build 5.31.743.005). Close

User manual
Getting started with UNICORN™ 5.31 Summary The purpose of Getting Started is to present a quick and easy guide to the system for a user with limited or no experience of UNICORN. The work flow is presented in the form of practical instructions for how to operate a model system. These instructions form a basic framework that the reader can expand on by reading selected parts in the other manuals. Close
Optional Column Selection Valve Summary Column and media screening can be automated by adding two motorized valves onto the system. This setup allows: Automated method optimization and multi-step purification as well as column selection. Up to eight columns can be connected onto the system. Column performance can be easily compared while changing conditions in a run. The setup is time saving and increases reproducibility of the results. Preset protocols will prevent over-pressurization and protect columns from breaking. Close
UNICORN™ 5.31 Administration and Technical Manual Summary This manual provides comprehensive information for network or UNICORN administrators, including network setup and configuration, software installation and maintenance and chromatography system setup, configuration and calibration. Close
UNICORN™ 5.31 Evaluation for Cross Flow Filtration / User Reference Manual Summary This manual describes the wizard that can be used for evaluation of cross flow filtration results in UNICORN 5.31. This manual refers to the use of ÄKTAcrossflow instruments and should be considered as a complement to the standard UNICORN 5.31 User Reference Manuals, 28997237AA and 28997239AA. Close
UNICORN™ 5.31 User Reference Manual Chapter 1 to 11 Summary The purpose of the User Reference Manual is to present a comprehensive guide to the UNICORN system for a user either with previous experience of this system or from other, similar chromatography systems. The system is presented in detail, along with practical instructions of how to operate a model system. The User Reference Manual is divided into two parts. The second part of this edition is 28997239AA, , containing chapters 12-15 and appendices. Close
UNICORN™ 5.31 User Reference Manual Chapter 12 to 15, Appendices Summary The purpose of the User Reference Manual is to present a comprehensive guide to the UNICORN system for a user either with previous experience of this system or from other, similar chromatography systems. The system is presented in detail, along with practical instructions of how to operate a model system. The User Reference Manual is divided into two parts. The first part of this edition is 28997237AA, , containing chapters 1-11. Close
ÄKTAFPLC™ Operating Instructions - Original instructions Summary The Operating Instructions provides you with the instructions needed to handle the ÄKTAFPLC system in a safe way. Close
ÄKTAdesign Buffer Selection Kit Summary ÄKTAdesign™ standard system configurations can be changed to optional configurations. This flexibility in ÄKTAdesign system strategies allows the user to enhance already used purification methods and also to develop new, more complex methods. When optional equipment is connected to a standard chromatographic system, new sets of instructions to control the optional equipment become accessible for the user. Optional configurations are selected, installed and put into operation by the user. An optional configuration consists of both hardware components and software instructions. Optional configurations are monitored and controlled via methods run by the UNICORN™ control system in the same way as ÄKTAdesign standard system configurations. Close
ÄKTAdesign Fractionation Kit Summary ÄKTAdesign™ standard system configurations can be changed to optional configurations. This flexibility in ÄKTAdesign system strategies allows the user to enhance already used purification methods and also to develop new, more complex methods. When optional equipment is connected to a standard chromatographic system, new sets of instructions to control the optional equipment become accessible for the user. Optional configurations are selected, installed and put into operation by the user. An optional configuration consists of both hardware components and software instructions. Optional configurations are monitored and controlled via methods run by the UNICORN™ control system in the same way as ÄKTAdesign standard system configurations Close
ÄKTAdesign Reverse flow Kit Summary ÄKTAdesign™ standard system configurations can be changed to optional configurations. This flexibility in ÄKTAdesign system strategies allows the user to enhance already used purification methods and also to develop new, more complex methods. When optional equipment is connected to a standard chromatographic system, new sets of instructions to control the optional equipment become accessible for the user. Optional configurations are selected, installed and put into operation by the user. An optional configuration consists of both hardware components and software instructions. Optional configurations are monitored and controlled via methods run by the UNICORN™ control system in the same way as ÄKTAdesign standardsystem configurations. Close

ÄKTAFPLC

Jump to: Accessories | Spare Parts | Top of Page

Accessories

Select to expand the desired collection of accessories.

For accessories not displayed in this section, please contact your local GE Healthcare sales representative.

#	Product Name	Product Code	Price
1	Fraction Collector Frac-950	18-6083-00		Quote
2	Fraction Collector Frac-920	18-1177-40	GBP 1,580	Add
3	Superloop, 1/16" fittings, 10 ml	18-1113-81	GBP 415	Add
4	Superloop, 1/16" fittings (ÄKTAdesign), 50 ml	18-1113-82	GBP 492	Add
5	Superloop Complete, 150 ml, M6 fitting	18-1023-85	GBP 703	Add
6	Short Column Holder, for columns 26 mm i.d.	18-1113-17	GBP 87.2	Add
7	Long Column Holder, columns 26 mm i.d.	18-1126-32	GBP 142.88	Add
8	Small Column Clamp	18-1149-98	GBP 15.23	Add

Jump to: Accessories | Spare Parts | Top of Page

Spare Parts

Select to expand the desired collection of spare parts.

Spare parts, front view

Spare parts not indicated in the front view

Unions, ferrules and stop plugs

Tubing

Spare parts recommended to keep on site

Accessory box delivered with the system

For spare parts not displayed in this section, please contact your local GE Healthcare service representative.

Spare parts, front view

Figure. ÄKTAFPLC, front view.

/gehcls_images/GELS/Images/TechSupportImages/Files/18114045_1.png

#	Product Name	Product Code	Price
1	Rinse Tubing	18-1032-11	GBP 36.93	Add
1	Pump head complete	18-1032-15	GBP 370	Add
1	Seal kit	18-1032-16	GBP 129.21	Add
1	Seal kit	18-1104-96	GBP 73.6	Add
1	Inlet tubing kit, i.d. 1.6 mm, o.d. 3/16 in	18-1119-26	GBP 61.53	Add
1	Internal tubing kit P-920	18-1128-70	GBP 218	Add
1	Cylinder	18-1512-01	GBP 143.11	Add
2	Filter 280 nm	18-0621-01	GBP 346	Add
2	Filter 214 nm	18-0622-01	GBP 359	Add
2	Filter 313 nm	18-0623-01	GBP 502	Add
2	Filter 365 nm	18-0624-01	GBP 486	Add
2	Filter 405 nm	18-0625-01	GBP 346	Add
2	Filter 436 nm	18-0626-01	GBP 438	Add
2	Filter 546 nm	18-0627-01	GBP 429	Add
2	Signal Cable, (6-pin miniDin-Open)	18-1110-64	GBP 118.72	Add
2	Dummy Electrode, round tip	18-1111-03	GBP 72.49	Add
2	Clamp, conductivity cell	18-1111-14	GBP 30.99	Add
2	pH Electrode, round tip	18-1111-26	GBP 509	Add
2	pH Flow Cell	18-1112-92	GBP 399	Add
2	Recorder REC-112 dual channel	18-1132-33
2	pH Electrode with Cell and Holder, round tip	18-1134-84	GBP 909	Add
2	Hg optics with 254 and 280 nm filters	28-4042-23	GBP 2,540	Add
2	Zn optics with 214 nm filter	28-4042-24	GBP 2,540	Add
3	Filter 254 nm	18-0620-01	GBP 340	Add
3	Conductivity Flow Cell, 1/16"	18-1111-05	GBP 663	Add
3	Flow cell holder	18-3055-87	GBP 151	Add
4	UV Flow Cell, 5 mm	18-1128-24	GBP 835	Add
4	UV Flow Cell, 2 mm	18-1128-25	GBP 618	Add
5	Motor Valve INV-907	18-1108-40	GBP 1,810	Add
5	Repair kit, valve INV-907	18-1109-05	GBP 306	Add
5	Number Plates 0–9	18-1109-09		Quote
5	Mounting Bracket for Valves	18-1109-11	GBP 11.56	Add
5	Injection Kit, INV-907	18-1110-89	GBP 218	Add
5	Fill Port, INV-907	18-1127-66	GBP 84.05	Add
6	Online filter kit	18-1027-11	GBP 51.48	Add
6	Online Filter Holder (20, 50, and 100 ml/min Systems)	18-1112-44	GBP 250	Add
6	On-line filter kit	18-1130-23	GBP 77.22	Add
7	Short Column Holder, for columns 26 mm i.d.	18-1113-17	GBP 87.2	Add
7	Long Column Holder, columns 26 mm i.d.	18-1126-32	GBP 142.88	Add
8	Flow Restrictor FR-904	18-1119-63	GBP 126.07	Add
8	Flow Restrictor FR-902	18-1121-35	GBP 202	Add
9	Stop Plug 1/16" Male	18-1112-52	GBP 116.62	Add
9	Mixer M-925 for ÄKTAdesign	18-1118-89	GBP 1,180	Add
9	Mixer M-925 Mixing Chamber, 0.6 ml	18-1118-90	GBP 293	Add
9	Mixer M-925 Mixing Chamber, 2 ml	18-1118-91	GBP 274	Add
9	Mixer M-925 Mixing Chamber, 5 ml	18-1118-92	GBP 292	Add
9	Mixer M-925 Mixing Chamber, 12 ml	18-1118-93	GBP 339	Add
10	Sample loop 100 µl	18-1113-98	GBP 87.2	Add
10	Sample loop 500 µl	18-1113-99	GBP 93.5	Add
10	Sample loop 1 ml	18-1114-01	GBP 99.81	Add
10	Sample Loop 2.0 ml, INV-907	18-1114-02	GBP 98.76	Add
10	Sample loop, 10 µl	18-1120-39	GBP 27.84	Add
10	Sample Loop 5 ml, PEEK	18-1140-53	GBP 93.19	Add
11	Hg lamp and housing complete	28-4042-25	GBP 652	Add
11	Zn lamp and housing complete	28-4042-26	GBP 653	Add
12	Inlet filter holder kit	11-0004-07	GBP 57.26	Add
12	Screw lid kit	11-0004-10	GBP 16.81	Add
12	Inlet filter set	11-0004-14	GBP 37.3	Add
12	Inlet Filter Assembly Kit	18-1113-15	GBP 72.49	Add
12	Inlet Filter Set	18-1114-42	GBP 65.66	Add
	Cable UniNet, L = 0.7 m	18-1109-74	GBP 87.2	Add

Spare parts not indicated in the front view

#	Product Name	Product Code	Price
1	Cable UniNet, L = 0.18 m	18-1109-72	GBP 44.65	Add
2	Cable UniNet, L = 0.3 m	18-1109-73	GBP 51.48	Add
3	Cable UniNet, L = 1.5 m	18-1117-75	GBP 99.81	Add
4	Cable UniNet, L = 3 m	18-1109-75	GBP 116.62	Add
5	Cable UniNet L=15 m	18-1117-74	GBP 180	Add
6	Mains cable, 120 V	19-2447-01	GBP 40.45	Add
7	Mains cable 220 V	19-2448-01	GBP 41.57	Add
8	Signal Cable, (6-pin miniDin-Open)	18-1110-64	GBP 118.72	Add
9	Small Column Clamp	18-1149-98	GBP 15.23	Add
10	Tubing Cutter	18-1112-46		Quote
11	Wrench, 1/4"	18-1112-45	GBP 44.38	Add
12	U-Wrench, M6	19-7481-01	GBP 34.83	Add
13	Allen key, 2.5 mm	19-4442-01	GBP 30.34	Add
14	Inlet tubing kit, i.d. 1.6 mm, o.d. 3/16 in	18-1119-26	GBP 61.53	Add

Unions, ferrules and stop plugs

#	Product Name	Product Code	Price
1	Fingertight Connector 1/16" Male for Tubing o.d. 1/16"	18-1112-55	GBP 51.48	Add
2	Connector 1/16" Male	18-1127-07	GBP 148.13	Add
3	Tubing connector 1/8"	18-1121-17	GBP 87.2	Add
4	Union 1/16" Female/M6 Male	18-1112-57	GBP 65.66	Add
5	Fingertight Union 1/16" Male/M6 Female	18-1112-58	GBP 79.85	Add
6	Connector 1/16" Male/Luer Female	18-1112-51	GBP 38.35	Add
7	Union 1/16" female-1/16" female	18-3855-01	GBP 69.66	Add
8	HiTrap/HiPrep, 1/16", male connector for ÄKTAdesign	28-4010-81	GBP 38.04	Add
9	Ferrules for Tubing, o.d. 1/16"	18-1127-06	GBP 59.88	Add
10	Ferrule for o.d.1/8" Tubing	18-1121-18	GBP 47.8	Add
11	Stop Plug 1/16" Male	18-1112-52	GBP 116.62	Add
12	Stop Plug 5/16" Male	18-1112-50	GBP 15.15	Add
13	Union 1/16" Male - 1/16" Male, i.d. 0.25 mm	18-1120-92	GBP 11.56	Add
14	Union 1/16" Male - 1/16" Male, i.d. 0.5 mm	18-1120-93	GBP 44.65	Add

Tubing

#	Product Name	Product Code	Price
1	Tubing i.d. 0.5 mm, o.d. 1/16"	18-1113-68	GBP 26.79	Add
2	Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16"	18-1112-53	GBP 38.35	Add
3	ETFE Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16 in	18-1119-74	GBP 26.79	Add
4	ETFE Tubing, 3 m, i.d.1.0 mm, o.d. 1/16 in	18-1142-38	GBP 30.6	Add
5	FEP Tubing, 3 m, i.d. 1/16 in, o.d. 1/8 in	18-1121-16	GBP 24.69	Add

Spare parts recommended to keep on site

Seals are considered as consumables and should be replaced annually or when needed. Please keep a set of each in your stock to prevent long downtime. For code numbers refer to the table below.

#	Product Name	Product Code	Price
1	Seal kit	18-1032-16	GBP 129.21	Add
2	Pump head complete	18-1032-15	GBP 370	Add
3	Rinse Tubing	18-1032-11	GBP 36.93	Add
4	Inlet filter set	11-0004-14	GBP 37.3	Add
5	Online filter kit	18-1027-11	GBP 51.48	Add
6	On-line filter kit	18-1130-23	GBP 77.22	Add
7	Flow Restrictor FR-902	18-1121-35	GBP 202	Add
8	Flow Restrictor FR-904	18-1119-63	GBP 126.07	Add
9	pH Electrode, round tip	18-1111-26	GBP 509	Add
10	Mixer M-925 Mixing Chamber, 0.6 ml	18-1118-90	GBP 293	Add
11	Mixer M-925 Mixing Chamber, 2 ml	18-1118-91	GBP 274	Add
12	Mixer M-925 Mixing Chamber, 5 ml	18-1118-92	GBP 292	Add
13	Mixer M-925 Mixing Chamber, 12 ml	18-1118-93	GBP 339	Add
14	Repair kit, valve INV-907	18-1109-05	GBP 306	Add

Accessory box delivered with the system

#	Product Name	Product Code	Price
1	Tubing Cutter	18-1112-46		Quote
2	Fingertight Connector 1/16" Male for Tubing o.d. 1/16"	18-1112-55	GBP 51.48	Add
3	HiTrap/HiPrep, 1/16", male connector for ÄKTAdesign	28-4010-81	GBP 38.04	Add
4	Tubing connector 1/8"	18-1121-17	GBP 87.2	Add
5	Ferrule for o.d.1/8" Tubing	18-1121-18	GBP 47.8	Add
6	Fingertight Union 1/16" Male/M6 Female	18-1112-58	GBP 79.85	Add
7	Union 1/16" Male - 1/16" Male, i.d. 0.5 mm	18-1120-93	GBP 44.65	Add
8	Connector 1/16" Male/Luer Female	18-1112-51	GBP 38.35	Add
9	Union 1/16" Female/M6 Male	18-1112-57	GBP 65.66	Add
10	Stop Plug 1/16" Male	18-1112-52	GBP 116.62	Add
11	Screw lid kit	11-0004-10	GBP 16.81	Add
12	Inlet filter set	11-0004-14	GBP 37.3	Add
13	Inlet filter holder kit	11-0004-07	GBP 57.26	Add
14	Online filter kit	18-1027-11	GBP 51.48	Add
15	Short Column Holder, for columns 26 mm i.d.	18-1113-17	GBP 87.2	Add

Share:

ÄKTAFPLC

ÄKTAFPLC

ÄKTAFPLC

ÄKTAFPLC

ÄKTAFPLC

Accessories

Spare Parts

Spare parts, front view

Spare parts not indicated in the front view

Unions, ferrules and stop plugs

Tubing

Spare parts recommended to keep on site

Accessory box delivered with the system