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PreScission Protease is a genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST. It specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro.
- Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence
- Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow
PreScission Protease is a genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST. This protease was specifically designed to facilitate removal of the protease by allowing simultaneous protease immobilization and cleavage of GST fusion proteins produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3; see pGEX Vectors (GST Gene Fusion System). PreScission Protease specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro.
PreScission Protease — Technical Specifications
|Complete Packsize||500 units|
|Application||Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors|
|Unit Definition||One unit will cleave > 90% of 100 µg of a test GST-fusion protein in 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 5°C for 16 h.|
|Vector||pGEX-6P-1, pGEX-6P-2, pGEX-6P-3|
|Molecular Weight [Mr]||46000|
|Inhibitor||100 mM ZnCl2 (< 50% inhibition); 100 µM chymostatin; 4 mM Pefabloc|
|Elution Buffer||50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0|
|Cleavage Buffer||50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), pH 7.0|
|Supplied As||2000 units/ml (833 to 1000 units/mg) in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM EDTA, 1 mM DTT, and 20% glycerol.|