PreScission Protease is a genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST. It specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro.

Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence
Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

PreScission Protease is a genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST. This protease was specifically designed to facilitate removal of the protease by allowing simultaneous protease immobilization and cleavage of GST fusion proteins produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3; see pGEX Vectors (GST Gene Fusion System). PreScission Protease specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro.

PreScission Protease — Technical Specifications

Complete Packsize	500 units
Application	Used for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors
Unit Definition	One unit will cleave > 90% of 100 µg of a test GST-fusion protein in 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.0 at 5°C for 16 h.
Vector	pGEX-6P-1, pGEX-6P-2, pGEX-6P-3
Molecular Weight [Mr]	46000
Inhibitor	100 mM ZnCl2 (< 50% inhibition); 100 µM chymostatin; 4 mM Pefabloc
Elution Buffer	50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Cleavage Buffer	50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), pH 7.0
Supplied As	2000 units/ml (833 to 1000 units/mg) in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 10 mM EDTA, 1 mM DTT, and 20% glycerol.