GST-Tagged Protein Purification
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Glutathione Sepharose chromatography media (resins) are available in lab packs, prepacked GSTrap and GSPrep columns, and 96-well plates. We also offer GST Purification Modules for purification of bacterial lysates. GST SpinTrap module includes 50 prepacked Glutathione Sepharose 4B SpinTrap columns as well as the buffers needed for purification. RediPack GST Purification Module includes gravity flow columns and buffer for purifying up to 20 mg protein.
Glutathione S-transferase (GST) is an enzyme found in most organisms. The functional protein is a dimer. The tag consists of 220 amino acid residues, making the tagged protein more soluble as well as simplifying its purification and detection. However, removal of GST tag from the target protein is often necessary due to its large size. This can be performed with PreScission Protease which is optimized for low-temperature cleavage.
The purification method is based on the high affinity of GST for glutathione. When applied to the affinity medium, GST-tagged proteins bind to the glutathione ligand, and impurities are removed by washing with binding buffer. Tagged proteins are then eluted from the chromatography medium under mild, non-denaturing conditions that preserve both protein structure and function. GST Buffer Kit contains prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins.
