Disposable PD-10 Desalting Columns
Disposable PD-10 Desalting Columns
Disposable PD-10 Desalting Columns
- Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex G-25 Medium.
- Flexible protocols allow runs by gravity flow or centrifugation.
- Easy to use and equilibrate; apply sample; elute.
- Preparation of the samples in the range of 1.0 to 2.5 ml by gravity flow and 1.75 to 2.5 ml with centrifugation.
- Desalt multiple samples in parallel with a desalting capacity > 90%.
- Recovery in the range 70 to >95%.
Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
Disposable PD-10 Desalting Columns — Technical Specifications
| Complete Packsize | 30 Columns |
| Bed Volume | 13.5 ml |
| Bed Dimensions | 14.5 x 50 mm |
| Sample Volume | 2.5 ml |
| Storage Conditions | 4 to 30°C, 0.15% Kathon CG/ICP Biocide |
| Wetted Part | No |
| Guidance |
Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 medium and for sample volumes up to 2.5 ml by gravity flow and columns spin flow 1 x Column stand 4 x PD-10 spin adaptor 1 x Buffer tray 30 x Bottom sleeve Instructions are supplied with the columns The white boxes in the picture are to secure safe transport of the columns PD- 10 Buffer reservoir has to be ordered separately For smaller scale please use PD MidiTrap G-25 (1.0 ml), PD MiniTrap G-25 (0.5 ml), PD SpinTrap G-25 (130 µl) and PD MultiTrap G-25 (130 µl in parallel) |
| Media | |
|---|---|
| BioProcess Media | Yes |
| Matrix | Cross-linked dextran |
| Particle Size | 85-260 µm |
| Partical Size Dry | >50 µm |
| pH stability Working Range | 2-13 |
| pH stability Cleaning | 2-13 |
| Pressure/Flow Specification | >100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d. |
| Fractionation [Mr] Globular Proteins | 1 x 103-5 x 103 |
| Fractionation [Mr] Dextrans | 1 x 102-1 x 102 |
| Exclusion Limit [Mr] [Globular Proteins] | > 5 x 103 |
| Exclusion Limit | Mr 5000 |
| Storage Conditions | 4 to 30°C, dry media. Used media 4 to 8 °C in 20% Ethanol or 0.1 M NaOH |
| Estimated Shelflife from Manufacture Date | 8 years |
| Chemical Stability | All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile |
| Column | |
|---|---|
| Column i.d. | 15 mm |
| Chemical Stability | Stable in all commonly used buffer systems |
| Material [Column Hardware] | Polypropylene (PP) |
| Material [Bed Support] | Polyethylene (PE) |
