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  • Chemiluminescent Labeling and Detection Reagents

    Since the early 1990s, we have continuously developed enhanced chemiluminescence detection systems, which are among the most widely used detection reagents for Western blotting applications today. GE Healthcare offers a variety of chemiluminescence detection reagents to cover a wide range of experimental needs.

    Enhanced chemiluminescent (ECL) detection is the most commonly used detection method in Western blot analysis. ECL is based on antibodies conjugated to horseradish peroxidase (HRP). HRP catalyzes the oxidation of luminol in the presence of peroxide, generating emission of low intensity light at 428 nm. The signal intensity is dependent of the number of HRP molecules and is proportional to the amount of antibody bound to the target molecule.


    Amersham ECL start
    Amersham ECL start Western Blotting Detection Reagent

    Chemiluminescent detection of medium- and high-expression proteins, with a long lasting signal that provides a longer time window for detection.

    Amersham ECL Western blotting detection reagent
    Amersham ECL Western Blotting Detection Reagent

    Reliable chemiluminescent detection of medium- and high expression proteins.

    Amersham ECL Prime Western Blotting Detection Reagent
    Amersham ECL Prime Western Blotting Detection Reagent

    Highly sensitive chemiluminescent detection reagent with long lasting signal for accurate detection of minute changes in protein levels.

    Amersham ECL Select Western Blotting Detection Reagent

    Very sensitive chemiluminescent detection reagent with highly intense signal output, for detection of medium to very low protein levels.

    Amersham ECL Western Blotting Reagent Pack

    Contains blocking and HRP-conjugated secondary antibodies. ECL detection reagent sold separately.

    ECL Western Blotting System

    ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.

    Amersham ECL Plus Western Blotting Reagents
    Amersham ECL Plus

    Amersham ECL Plus has been discontinued - switch to Amersham ECL Prime today

    Amersham ECL Glycoprotein Detection Module

    Nonradioactive labeling of carbohydrate moieties on proteins and detection using streptavidin-horseradish peroxidase and ECL reagents.

    Amersham ECL GST Western Blotting Detection Kit

    Sensitive and convenient detection of Glutathione S-transferase (GST) fusion proteins on western blots using chemiluminescence with ECL detection reagents.

    Anti-GST HRP Conjugate

    This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents.

    Amersham ECL Protein Biotinylation Module/System

    Protein biotinylation and detection using streptavidin-HRP and ECL reagents.

    Anti-His Antibody
    Anti-His Antibody

    Anti-His Antibody is an IgG₂ subclass of monoclonal antibody directed against six histidine residues, which are commonly used as an affinity tag for recombinant fusion proteins.

    Anti-GST Antibody
    Anti-GST Antibody

    Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST, Because of its polyclonal nature, it can recognize more than one epitope on GST.

    Biotinylated Antibodies

    The antibodies are supplied with dilution guidelines for relevant applications.