ÄKTAprime plus is a compact one-step purification system that operates with most prepacked columns. For reliability and convenience, we recommend HiTrap, HiPrep or HiLoad columns that simply snap on to the side of the unit. The system has preprogrammed methods for typical applications such as purification of affinity tagged proteins, MAbs and sample clean-up. The flexible system can be used for more general purification tasks such as buffer exchange, gel filtration, ion exchange, HIC, and affinity chromatography.

The unit is compact and comprises a system pump, fraction collector, monitors for UV, conductivity and pH (optional). Valves for buffer selection, sample injection, gradient formation, and flow diversion are also integrated into the system. The system is not PC controlled but together with PrimeView software it offers continuous real-time monitoring and evaluation of results.

ÄKTAprime plus — Technical Specifications

Complete Packsize	1 Piece
Order Information	Excluding recorder
Application	Simple purification of tagged and untagged proteins
Flow rate	0.1 ml/min-50 ml/min
Flow Rate	0.1 ml/min-50 ml/min
Operating Pressure Max.	1 MPa (145 psi)
Tubing i.d. [Flow Path]	0.75 mm
Single Wavelength Detection	Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm. Optional zink lamp with 214 nm filter.
Multiple Wavelength Detection	No
Flow Cell Path Length	2 mm (optional 5 mm)
pH Monitoring	0-14
Multiple Sample Injection	Optional, up to 2 samples
Autosampler Injection	No
Automatic Buffer Preparation	No
Buffer Selection	2 buffer inlets
Multi-Step Purification	Optional, up to 2 steps
Relative Humidity	20%-95% (non condensing)
pH Stability Long Term	2-12
pH Stability Short Term	0-14
Viscosity	max 10 cP (<10 ml/min), max 5 cP (>10 ml/min)
Width	400 mm
Height	530 mm
Depth	450 mm
Weight	13 kg
Voltage	100-120/220-240 VAC
Protection Class	IP 20 (Housing) IP 44 (Flow cell)
Compliance	The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended. For regulatory details please see declaration of conformity.

Animation
HiTrap: Connecting to a chromatography system (ÄKTA) Close

Brochure
Instrument Services for ÄKTA Systems Summary ÄKTA design is a platform of systems that gives you a competitive advantage in biomolecule purification and separation. Our service engineers are specifically trained to keep your ÄKTA system performing optimally, to save you time and maximize productivity, allowing you to rely on your results and concentrate on your research. Close
Protein purification - at the touch of a button Summary ÄKTAprime plus is a compact liquid chromatography system designed for one-step purification of proteins. Many protein purification activities can be carried out just by pressing a button. The system is pure simplicity to operate, with everything controlled from push buttons and an easy-to-navigate LCD display on the front panel. With ÄKTAprime plus, reliable and convenient laboratory scale protein purification is no longer pure imagination. Close
Validation Services Summary GE Healthcare Validation Services supports efficient validation of your GE Healthcare equipment compliance status. Our documentation supports your equipment throughout its entire life cycle. When you choose GE Healthcare as your provider of validation support, you can be confident that your equipment is operating in a reliable, consistent, and GxP-compliant manner. Close
ÄKTA Protein purification by design Summary With the ability to purify virtually any biomolecule, ÄKTA design platform can handle the simplest and the toughest of challenges. It gives you speed, ease of use, and flexibility whatever your purification application or scale. The platform covers all major chromatographic and cross flow filtration techniques, from the research laboratory to process development and manufacturing. Systems in the ÄKTA platform work with intelligent UNICORN software, which makes it simple to control every stage of your purification process. A broad range of prepacked columns, media, and filters provides more options for optimum results. Close

Cue card
Strep(II)-tagged protein purification - ÄKTAprime™ Plus Close
ÄKTAprime plus Summary Contents System Preparation Buffer exchange on HiTrap Desalting Buffer exchange on HiPrep 26/10 Desalting Histidine-tagged protein purification,step elution Histidine-tagged protein purification, gradient elution IMAC purification - any metal On-column refolding GST-tagged protein purification Strep(II)-tagged protein purification MBP-tagged protein purification MAb purification, step elution MAb purification, gradient elution IgM purification Albumin removal Removal of trypsin-like serine proteases Anion exchange Cation exchange Method Templates value table Close

Cue card

Strep(II)-tagged protein purification - ÄKTAprime™ Plus

ÄKTAprime plus Summary

Handbook
Affinity Chromatography, Principles & Methods Summary This handbook describes the role of affinity chromatography in the purification of biomolecules, the principle of the technique, the media available and how to select them, application examples and detailed instructions for the most commonly performed procedures. Practical information is given as a guide towards obtaining the best results. Close
Gel Filtration, Principles and Methods Summary Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. Separations can be performed in the presence of essential ions or cofactors, detergents, urea, guanidine hydrochloride, at high or low ionic strength, at 37 °C or in the cold room according to the requirements of the experiment.This handbook describes the use of gel filtration for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date gel filtration media and prepacked columns.The biocompatibility, stability and utility of gel filtration media from Amersham Biosciences have made these products the standard choice in practically every laboratory using the technique. A wide variety of prepacked columns and ready to use media is available. Close
Ion Exchange Chromatography & Chromatofocusing, Principles and Methods Summary Ion exchange chromatography (IEX) has been used for separating biomolecules since the 1960s and continues to play a major role in the separation and purification of biomolecules. Today, IEX is one of the most requently used techniques for purifying proteins, peptides, nucleic acids and other charged biomolecules, offering high resolution and group separations with high loading capacity. The technique is capable of separating molecular species that have only minor differences in their charge properties, for example two proteins differing by one charged amino acid. These features make IEX well-suited for capture, intermediate purification, or polishing steps in a purification protocol and the technique is used from microscale purification and analysis through to purification of kilograms of product. Close
Purifying Challenging Proteins - Principles and Methods Summary This handbook is intended for students and experienced researchers with an interest in the isolation of integral membrane proteins, multiprotein complexes, or in refolding proteins from inclusion bodies. The aim is to present tools, strategies, and solutions available to meet the purification challenges associated with these three classes of proteins. Close
ÄKTA Laboratory-scale Chromatography Systems - Instrument Management Handbook Summary This handbook is focused on liquid chromatography systems used for protein purification at research laboratory scale. Beginners can use the handbook to obtain an overview of how purification systems work and to learn about important considerations for achieving successful results. Experienced system users will also find valuable and detailed information on different hardware modules. Close

Instruction/protocol
UV lamp exchange Summary This instruction is valid for the Hg and Zn lamps. Close
Valve INV-917 Summary Valve INV-917 is a motorised rotary 7-port valve. The valve is used in ÄKTA™ design systems. It is powered from P-900 series system pump, and controlled from UNICORN™ control system. The valve has 3 positions used for: - loading a sample loop - injecting the sample onto the column - washing the system pump. In ÄKTAmicro™ system, INV-917 is also used as a Flow direction valve. Features: • Smooth flowpath minimises eluent or sample ”memory effect”. • Flow rates up to 10 ml/min. • All wetted parts are plastic PEEK. Close

Instruction/protocol

UV lamp exchange Summary

Valve INV-917 Summary

Magazine
Discovery Matters 5-2007 Summary Table of contents - Optimizing Ad-A-Gene assay parameters - Two-step purification of a challenging membrane protein - Enrichment of protein prior to LC-MS analysis - Rapid purification of plasmid DNA Close
Discovery Matters 6-2007 Summary Table of contents - Phi29 DNA polymerasebased technology for genomic DNA preparation - Identifying chromosomal abnormalities in prostate cancer - Multiplex assays using Ad-A-Gene Vectors - Multiplex detection in Western blotting - Chromatographic analysis of glycoproteins Close

Magazine article
Low-cost, automated serial purification of histidine-tagged proteins (Discovery Matters 6, Aug 2007) Summary Automated chromatographic systems are of great help to structural genomics programs involved in expressing and purifying numerous recombinant proteins. A low-cost and automated process, using ÄKTAprime™ chromatography system is described for the purification of histidine-tagged proteins. Close
Monitoring expression of an E. coli membrane protein GFP-(His)8 fusion and purification using a combination of IMAC and gel filtration Summary An integral E. coli membrane protein GFP-(His)8-fusion was overexpressed, solubilized, and purified to homogeneity. The GFP-moiety allowed direct monitoring of the protein during all steps from expression to purification, while the histidine tag expressed on the protein facilitated capture of the protein of interest by immobilized metal ion affinity chromatography (IMAC) on a HisTrap™ HP column. Close

Magazine article

Low-cost, automated serial purification of histidine-tagged proteins (Discovery Matters 6, Aug 2007) Summary

Monitoring expression of an E. coli membrane protein GFP-(His)8 fusion and purification using a combination of IMAC and gel filtration Summary

Quality system certificate
Certificate for ÄKTAprime plus (example) Close

Software
Downloadable installation program for USB-driver update for existing PrimeView 5 Installations Summary The driver improves the capabilty to use USB converters for connecting the ÄKTAprime plus to the PrimeView 5 software. Click on the file to download. Close

Software change description
PrimeView v5.0 vs 1.0, Software change description Close

User manual
Fraction Collector Frac-950 Summary This manual comprises two parts: a practical part (sections 1–5) and a reference part (sections A-D). Sections 1–5 contain the necessary information for operating the instrument. Fraction Collector Frac-950 is an automated fraction collector for use in ÄKTA™ design chromatography systems. It is intended to be operated as an integrated part of an ÄKTAdesign chromatography system running UNICORN™ version 3.2, or higher. Frac-950 is equipped with an accumulator to eliminate spillage at high flows. A drop sensor that can be used to control tube change at low flows is also included. Close
PrimeView 5.0 Summary PrimeView™ is a complete control software package for supervision of ÄKTAprime™ automated liquid chromatography systems. Close
ÄKTAprime™ plus Operating Instructions - Original instructions Summary The Operating Instructions provides you with the instructions needed to handle the ÄKTAprime plus system in a safe way. Close

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Accessories

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ÄKTAprime plus kit

Accessories for ÄKTAprime plus

For accessories not displayed in this section, please contact your local GE Healthcare sales representative.

ÄKTAprime plus kit

#	Product Name	Product Code	Price	Qty
1	ÄKTAprime plus Kit	28-9348-28	GBP 125.33		Add

Accessories for ÄKTAprime plus

#	Product Name	Product Code	Price
1	Superloop, 1/16" fittings, 10 ml	18-1113-81	GBP 415	Add
2	Superloop, 1/16" fittings (ÄKTAdesign), 50 ml	18-1113-82	GBP 492	Add
3	Superloop Complete, 150 ml, M6 fitting	18-1023-85	GBP 703	Add
4	Recorder REC-112 dual channel	18-1132-33

Jump to: Accessories | Spare Parts | Top of Page

Spare Parts

Select to expand the desired collection of spare parts.

Spare parts, front view

Spare parts not indicated in the views

Spare parts for pump P-960

Spare parts for UV, pH and conductivity recording

Spare parts for Fraction collector

Spare parts for IV-908

Spare parts for Injection valve INV-907

Spare parts, Accessory box delivered with the system

Spare parts recommended to keep on site

Tubing

Unions, ferrule and stop plug

For spare parts not displayed in this section, please contact your local GE Healthcare service representative.