Expression of Recombinant Proteins
GE Healthcare Life Sciences offers a range of expression vectors for common tags directed towards both localizing expression products in whole cells and simplifying purification of recombinant proteins. Recombinant products including Green Fluorescent Protein (GFP) or related proteins can be visualized using IN Cell Analyzer for high content analysis. Purification of recombinant proteins is supported by a range of media and methods for affinity chromatography.
Recombinant proteins have become increasingly common, as has the wealth of techniques and products used for their creation, amplification, and purification. The advantages of using a recombinant tagged protein to facilitate purification and detection are now widely recognized. Many host systems are available including bacteria, yeast, plants, filamentous fungi, insect or mammalian cells grown in culture, and transgenic animals or plants. Different hosts are appropriate for different vectors, and each host system has its own advantages and disadvantages, which are important to consider before the final selection. Expression of recombinant proteins can also be exploited to investigate the localization of proteins within whole cells, by incorporating fluorescent markers such as GFP in the recombinant construct.
The localization and functions of tagged recombinant proteins expressed in vivo provide important biological insights
Efficient expression and purification of recombinant proteins is a prerequisite in many protein studies. Efficient isolation and purification of recombinant proteins is a corner stone in modern protein research.