Nucleic Acid Isolation and Purification
GE Healthcare Life Sciences offers a range of kits for you to extract and purify genomic DNA, plasmid DNA, mRNA, and total RNA from a range of starting materials. Our kits, columns, and filtration media enable DNA clean-up after manipulation techniques. Kits are also available to collect, archive and purify DNA, and to amplify whole genomes and plasmids.
Isolation of pure nucleic acids is a critical starting point for methods in molecular biology. For example, genomic analysis demands purification of highly pure DNA. Analysis of gene expression requires mRNA isolation with effective elimination of degrading RNases. Manipulation and analysis of nucleic acids involves moving them between different environments, which demands removal of contaminating and disturbing reaction components between steps.
DNA and RNA purification kits must therefore be able to handle a variety of target molecules and a vast array of cellular sources, from human tissues down to bacterial cells. The amount of nucleic acid must be sufficient for the next step. High purity improves stability and minimizes disturbance from non-target nucleic acid and other contaminants, such as proteins and small molecules. The success of your downstream applications therefore depends on rapid, reliable, and efficient DNA or RNA extraction and purification.
The purification of DNA, either genomic or as a plasmid, is frequently a critical step in molecular biology, clinical research, genomics, or biotechnology.
Manipulating nucleic acids almost always demands an effective cleanup before the next step
Your choice of a rapid and effective method for RNA isolation is critical since RNA is susceptible to degradation by ubiquitous RNases