Nucleic Acid Synthesis and Amplification
GE Healthcare Life Sciences offers instruments and reagents for chemical synthesis of oligonucleotides for research and therapeutic applications. Kits and enzymes cover your needs for cDNA synthesis and amplification by PCR, and incorporation of fluorescent labels into probes. We also have kits that exploit rolling circle amplification to prepare circular sequencing templates or mass-copy genomic DNA.
DNA synthesis and amplification enable genetic regions to be copied, located, identified, and manipulated in a myriad of ways. These methods, based on synthesis through organic chemistry or the action of polymerases, have been vital in the recombinant DNA and genomics revolutions and remain central to modern molecular biology. The accurate synthesis of oligonucleotides is a prerequisite for amplification methods such as PCR and many other techniques in molecular biology. The synthesis of DNA from RNA, followed by DNA amplification, has greatly simplified the analysis of gene transcription products (mRNA). Novel techniques employing the ability of viral polymerase to amplify circular DNA have led to significant advances in sequencing technologies. These techniques will play a major role in emerging areas such as nucleic acid-based therapies and pharmacogenetics.
Nucleic acid amplification is a powerful technique for producing large quantities of genetic regions, yielding amounts sufficient for a variety of methods.
Nucleic acid synthesis provides fundamental components for many molecular biology methods