DNA Modification and Cloning
GE Healthcare Life Sciences offers a range of enzymes to perform some of the most common modifications of nucleic acids, as well as a wide range of solutions for the purification, storage, and amplification of nucleic acid samples. Cloning is supported by general purpose cloning vectors and convenient kits for PCR-based cloning strategies.
Molecular cloning involves inserting an isolated DNA molecule into a suitable vehicle (vector) for transfer into a host cell for propagation. Cloning can be used to generate large numbers of copies of DNA for sequencing, or in the form of gene cloning to express genes in a variety of host organisms. Cloning was one of the first techniques to demand extensive enzymatic modification of DNA. Since then other techniques have extended and refined methods for manipulation of both DNA and RNA. One method of note is the Polymerase Chain Reaction (PCR) and the combination of PCR with reverse transcription to reverse-engineer expressed genes. The primary methods for enzymatic modification involve cleavage at specific positions, modification of fragment ends, including addition of groups such as labels, and linking fragments together.
A number of the methods you use in molecular biology will require enzymatic modification of DNA.
Successful cloning of your nucleic acid fragment involves a number of steps and reagents