Protein Sample Preparation
Initial protein extraction is often based on buffers containing mild non-ionic detergents, various salts, stabilizing agents, chelating agents, reducing agents, and protease inhibitors.
The choice of protease inhibitor may be critical to maintaining yield, structure, and function. Nucleases can be used to remove nucleic acids, and care should be taken to remove contaminants, including detergents that might interfere with downstream applications.
The preparation of protein samples is critical to the success of analytical methods such as mass spectrometry, 2-D gel electrophoresis, 1-D SDS-PAGE, trypsin digestion, and chromatography.