Samples can be extracted from the FTA card into solution for downstream analysis. DNA extraction methods may be reagent-based (e.g., chelating resin or organic extraction), or kit-based. The ease of handling of FTA cards, with physical attachment of sample information, such as barcodes, facilitates high-throughput sample processing using semi-automated and automated punching systems.
Quick and Flexible Sample Processing
The use of FTA for DNA collection enables your laboratory to reduce processing time while maintaining data quality. Punches from blood and buccal samples collected on FTA cards or EasiCollect can be processed for direct STR amplification chemistries in 5 minutes. If speed is not a concern, samples can be processed with standard chemistries in 30 minutes. For additional flexibility, DNA can be extracted from FTA using several commercially available extraction reagents and purification kits.
Manual and Semi-Automated Punching
GE Healthcare offers several manual and semi-automated punching tools that address your laboratory's punching requirements. Two different manual punch tools are available for use with FTA cards—Harris Micro-Punch and Harris Uni-Core Punch. The Harris Uni-Core and Micro-Punch products are designed with a very sharp, stainless steel cutting edge. The Harris e-Core tool is a compact, versatile, electric coring instrument designed for unrestricted sampling of FTA and FTA Elute cards. Harris e-Core utilizes 1.2, 2.0, and 3.0 mm replaceable Harris Micro-Punch Tips.
Choose from a range of cards and kits including FTA Cards and FTA Elute Cards
Disposable punch that provides up to 500 punches
Punch with replaceable steel tip that provides up to 2000 punches
Compact electric coring instrument designed for unrestricted sampling of FTA cards
Absorbent material designed to protect laboratory surfaces