Western Blotting
Western Blotting
Related Technologies
Western blotting is a widely used technique for the detection and analysis of proteins. It was first described in 1979 and has since become one of the most commonly used methods in life science research.
Western blotting is frequently used to study single proteins but the advent of multiplexed, fluorescence-based detection enables the simultaneous detection of several proteins. From routine protein detection to advanced triple detection or 2-D Western Blotting, GE Healthcare offers products for your entire workflow.
The steps for Western blotting are: Sample Preparation, Electrophoresis, Blotting, Antibody Labeling and Probing, and Detection and Imaging.
In Western blotting, native or denatured proteins are first separated by gel electrophoresis according to polypeptide length (under denaturing conditions) or by the three-dimensional structure of the protein (under native/non-denaturing conditions). The separated proteins are then transferred to a membrane, typically made of nitrocellulose or polyvinylidine difluoride (PVDF), where they are probed using antibodies specific to the target protein.
Western blotting is used for both qualitative and quantitative analysis of protein expression in a wide variety of biological samples, such as tissue homogenates or cultured cells.
Western blotting products from GE Healthcare have a reputation for high quality results, and some of our brand names such as ECL, Hyperfilm and Hybond, are among the most frequently cited in the scientific literature relating to Western blotting. Join our Western Blotting Discussion Forum to connect with our specialists and share valuable tips with colleagues around the world.
