One late night during 1957, as a young scientist was leaving the lab, he made a mistake. The mistake would be a turning point for his professor, Jerker Porath, and lead to the remarkable discovery of Sephadex (SEparation PHArmacia DEXtran).
Because the young scientist forgot to turn on the power switch when he left the lab that night, Jerker Porath realized that a gel made of crosslinked dextran could be used to separate macromolecules—without applying electrical current. The molecular separation was based purely on size.
The crosslinked dextran would ultimately become Sephadex, the first chromatography medium, and the technique would be referred to as gel filtration or size exclusion chromatography. Sephadex was an instant success, enabling new scientific disciplines and paving the way for the biopharmaceutical industry. Since then, GE Healthcare has developed numerous products for biomolecular separations based on Sephadex.
Sephadex is recommended at laboratory and production scale for rapid desalting, buffer exchange, and sample clean-up.
- Wide selection of exclusion limits: broad range of media for removing contaminants such as salts, dyes, and radioactive labels.
- High-recovery desalting and buffer exchange: high quality group separation of different sized biomolecules.
- Prepacked in a range of formats: extensive range of prepacked column and plate formats for convenience and reproducibility.
- Well-proven: widely used in laboratory and industrial applications for more than 50 years.
Lab packs of Sephadex gel filtration chromatography media for desalting, buffer exchange, and cleanup.
Prepacked, disposable desalting columns for buffer exchange and sample cleanup.
Prepacked HiTrap 5-ml columns for convenient desalting and buffer exchange.
Prepacked HiPrep 53-ml column for desalting and buffer exchange.
Prepacked filter plates for high throughput desalting and buffer exchange of multiple samples.