HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

34 µm bead size for high-performance, high-resolution purifications.
Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
Designed for use with a syringe, peristaltic pump, and chromatography systems such as ÄKTA design.
Strong sulfopropyl (SP) cation exchanger.

The small particle size (34µm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

HiTrap SP HP — Technical Specifications

Bed Volume	1 ml
Bed Dimensions	7 × 25 mm
Flow Rate	< 4 ml/min¹⁾
Flow rate	<4 ml/min²⁾
Storage Conditions	4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
Pressure Max. [Over the Packed Bed During Operation]	3 bar [0.3 MPa] (42 psi)
¹⁾The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
²⁾The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Media

BioProcess Medium	Yes
Ligand	Sulphopropyl
Average Particle Size	34 µm
Ion Exchanger Type	Strong cation exchanger
Matrix	6% cross-linked agarose
Particle Size	24 µm-44 µm
Ionic Capacity	0.15-0.20 mmol H⁺/ml
pH stability Working Range	4-13
pH Stability Working Range	4-13
pH Stability Cleaning	3-14
pH stability Cleaning	3-14
Pressure/Flow Specification	150 cm/h (at < 3 bar, 25°C, 10 cm bed height)
Exclusion Limit [Mr] [Globular Proteins]	4 x 10⁶
Storage Conditions	4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
Estimated Shelf Life from Manufacture Date	5 years
Chemical Stability	Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS¹⁾
Charged Groups	- SO₃^-
Dynamic Capacity	55 mg ribonuclease/ml medium
Efficiency	> 12 000 m^-¹
¹⁾1 M NaOH and acetic acid should be used only for cleaning purposes.

Column

Complete Packsize	1 ml
Column i.d.	7 mm
Material [Column Hardware]	Polypropylene (PP)

Animation
HiTrap: Connecting to a chromatography system (ÄKTA) Close
HiTrap: To use with a syringe Close
Protein purification strategy (Capture, Intermediate Purification, Polishing) Close
The principle of Ion Exchange Chromatography Close

Brochure
Protein purification - Applications that meet your needs Summary You must deliver quality results, pure protein targets and publish findings—in time and on budget. While it is complex work, purification need never to be an obstacle for you. Better still, it offers you opportunities to gain real competitive advantages. Here you’ll find proven solutions to many of today’s protein purification challenges. As well as an overview of the chromatography systems, services and quality assurances that we make available to support your science. With nearly 50 years of experience and a wealth of knowledge in purifying proteins, the Protein Separations specialists at GE Healthcare are ready to help you gain real competitive advantages. (40 pages) Close
ÄKTAxpress - Take advantage of your routines; be in two places at once Summary ÄKTAxpressTM is a complete, intelligent, and robust solution for multidimensional purifi cation of affinity-tagged proteins and antibodies. Automated multistep purifi cation provides the highest possible protein purity. ÄKTAxpress can run multiple samples providing both the required purity and high throughput. Robotic systems are limited to a single chromatography step and sacrifice purity to achieve high throughput. Now it is possible to purify proteins expressed at all levels, even proteins expressed at low levels can be purified. Dual-tagged proteins are purified easily and you have the option of on- or offcolumn tag cleavage. Close

Brochure

Protein purification - Applications that meet your needs Summary

ÄKTAxpress - Take advantage of your routines; be in two places at once Summary

Data file
Q & SP Sepharose High Performance Summary Q Sepharose High Performance and SP Sepharose High Performance enjoy well-deserved reputations as highly successful anion and cation ion exchange media for purifying a wide range of biomolecules. Both share an impressive list of operational characteristics that includes: High-resolution, high-capacity separations with high recovery Excellent flow rates Reliable and reproducible High chemical stability for effective CIP/sanitization Available in convenient HiLoad and HiTrap prepacked columns plus laboratory packs Easy scale-up With these attributes, Q and SP Sepharose High Performance occupy a central position in the broad spectrum of GE Healthcare ion exchange products. Their high resolution generates distinct, high purity separations and their high capacity and ease of use encourages preparative use and scale-up, primarily in intermediate and final purification. Availability in different prepacked HiPrep and HiTrap column formats, plus laboratory packs, gives users the freedom to enjoy the convenience and simplicity of ready-to-use columns and the flexibility of choosing column type and size. Close

Data file

Q & SP Sepharose High Performance Summary

Handbook
Ion Exchange Chromatography & Chromatofocusing, Principles and Methods Summary Ion exchange chromatography (IEX) has been used for separating biomolecules since the 1960s and continues to play a major role in the separation and purification of biomolecules. Today, IEX is one of the most requently used techniques for purifying proteins, peptides, nucleic acids and other charged biomolecules, offering high resolution and group separations with high loading capacity. The technique is capable of separating molecular species that have only minor differences in their charge properties, for example two proteins differing by one charged amino acid. These features make IEX well-suited for capture, intermediate purification, or polishing steps in a purification protocol and the technique is used from microscale purification and analysis through to purification of kilograms of product. Close
Purifying Challenging Proteins - Principles and Methods Summary This handbook is intended for students and experienced researchers with an interest in the isolation of integral membrane proteins, multiprotein complexes, or in refolding proteins from inclusion bodies. The aim is to present tools, strategies, and solutions available to meet the purification challenges associated with these three classes of proteins. Close
Strategies for protein purification Summary Strategies for Protein Purification is focused on chromatographic purification of proteins at research scale. Beginners can use the handbook to get a quick start when entering the field; experienced protein purifiers may also find valuable information. The handbook describes how to design a protein purification procedure, how to select and combine chromatography methods and purification formats, and how to monitor and evaluate protein purification. It gives an overview of the available methods and provides advice on how to avoid pitfalls in all operations, from initial sample preparation to final analysis of the purified protein. Close

Instruction/protocol
HiTrap SP & HiTrap Q HP, 1 ml and 5 ml Summary HiTrap™ SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules. HiTrap SP HP and HiTrap Q HP provide fast, reproducible, and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign™ or FPLC™ System. Close

Instruction/protocol

HiTrap SP & HiTrap Q HP, 1 ml and 5 ml Summary

Magazine
Discovery Matters issue 14 Summary Discovery Matters is a quarterly magazine from GE Healthcare with a wide range of articles covering topics relevant to the life science researcher. This issue (issue 14, October 2011) contains articles on: 1) Automated purification of antibody fragments using protein A and G prepacked gravity-flow columns 2) Multistep purification of tagged proteins for surface-plasmon resonance studies using ÄKTAxpress™ 3) The use of high-content analysis to develop a high-throughput wound healing assay for cancer research 4) Comparative performance of Amersham™ Hyperfilm™ Blue film for visualization of proteins The What’s New Section covers the major launches during Q2 and Q3 2011. Close

Magazine

Discovery Matters issue 14 Summary

Magazine article
Multistep purification of humanized WO-2 Fab using ÄKTAxpress™ for crystallization studies- Article from Discovery Matters issue 14 Summary ÄKTAxpress is a suitable chromatography system for the unattended purification of poorly expressed histidine-tagged proteins from E. coli lysates. A single purification method was designed to incorporate four chromatography techniques (affinity, desalting, ion exchange, and gel filtration [size exclusion chromatography]) without the need for operator intervention. The resultant protein, humanized WO-2 Fab antibody fragment, was purified to a high degree of purity in a single day and subsequently crystallized overnight. These results demonstrate the opportunity to streamline protein purification schemes by combining single methods into multistep methods using an ÄKTAxpress system. We were able to diminish the bottleneck in our protein production workflow and convert protein from E.coli lysate to protein crystal in two days Close
Multistep purification of recombinant tagged proteins for SPR studies using ÄKTAxpress™ - article from Discovery Matters 14, October 2011 Summary Recombinant tagged DNaseE7 and NC10 scFv-Im7, used for the development of a novel protein capture assay based on surface plasmon resonance (SPR) technology, were purified on ÄKTAxpress™ system from E. coli lysates. Single-step methods were replaced by unattended multistep methods, incorporating multiple chromatography techniques: Affinity (IMAC or a customized affinity chromatography [AC] column), desalting (DS), ion exchange chromatography (IEX), and gel filtration (GF). An overnight dialysis step into a low pH buffer was successfully replaced by a DS step into the same buffer, automating the purification. These methods, incorporating operator predefined UV280 peak collection parameters, have enabled us to rapidly and reliably purify the recombinant tagged proteins. Close
Redesigned HiTrap fittings offer easier connection to ÄKTAdesign chromatography systems (Life Science News 18, p19) Summary The new inlet and outlet design of HiTrap columns eliminates the need for column connectors when used with ÄKTAdesign™ chromatography systems. Connection to a syringe, laboratory pump, or chromatography system such as FPLC™ System is also possible using the provided connectors. The guide on this page shows the different types of connector and explains their use. Close
Strategies for antibody purification Close

Material Safety Data Sheet
HiTrap SP 1 ml, 5 x 1 ml Close

Reference list
HiTrap SP HP and HiTrap SP FF Summary The reference list for this product. Close

Selection guide
Guide sheet ÄKTAFPLC Summary A selection guide which shows the prepacked columns designed to work with ÄKTAFPLC Close
Ion exchange columns and media - Selection guide Summary This selection guide provides general guidelines, based on purification scale and sample conditions. Additional information is found in the Ion Exchange, Principles and Methods, Handbook available from GE Healthcare. Close
Prepacked chromatography columns for ÄKTA™ design systems Summary Selection guide describing all the prepacked columns available from GE Healthcare for use with ÄKTA™ chromatography systems. Close
Prepacked columns designed to work with ÄKTAxpress Summary ÄKTAxpress™. Unattended purification of tagged proteins and antibodies. Prepacked columns designed to work with ÄKTAxpress. Close
Protein and Peptide Purification: Technique selection guide Summary This technique selection guide is designed to help you in selecting the technique most suited to your application. It covers ion exchange chromatography (IEX), reversed phase chromatography (RPC), hydrophobic interaction chromatography (HIC), gel filtration (GF), affinity chromatography (AC). Close

Jump to: Accessories | Top of Page

Accessories

Select to expand the desired collection of accessories.

Running HiTrap, HisTrap and GSTrap with a stand alone pump

Running HiTrap, HisTrap and GSTrap with a syringe

Running HiTrap, HisTrap and GSTrap with ÄKTAdesign systems

For accessories not displayed in this section, please contact your local GE Healthcare sales representative.

Running HiTrap, HisTrap and GSTrap with a stand alone pump

/gehcls_images/GELS/Images/TechSupportImages/Files/18114736_46.png

#	Product Name	Product Code	Price
1	Tubing Cutter	18-1112-46		Quote
1	Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16"	18-1112-53	USD 55.68	Add
1	Tubing i.d. 0.5 mm, o.d. 1/16"	18-1113-68	USD 45.18	Add
1	Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16"	18-1115-83	USD 47.28	Add
1	Tubing i.d. 0.25 mm, o.d. 1/16"	18-1120-95	USD 38.87	Add
2	HiTrap/HiPrep, 1/16", male connector for ÄKTAdesign	28-4010-81	USD 69.29	Add

Running HiTrap, HisTrap and GSTrap with a syringe

/gehcls_images/GELS/Images/TechSupportImages/Files/18114736_47.jpeg

#	Product Name	Product Code	Price	Qty
1	Connector 1/16" Male/Luer Female	18-1112-51	USD 61.99		Add

Running HiTrap, HisTrap and GSTrap with ÄKTAdesign systems

/gehcls_images/GELS/Images/TechSupportImages/Files/18114736_45.png

#	Product Name	Product Code	Price
1	Tubing Cutter	18-1112-46		Quote
1	Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16"	18-1112-53	USD 55.68	Add
1	Tubing i.d. 0.5 mm, o.d. 1/16"	18-1113-68	USD 45.18	Add
1	Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16"	18-1115-83	USD 47.28	Add
1	Tubing i.d. 0.25 mm, o.d. 1/16"	18-1120-95	USD 38.87	Add
2	HiTrap/HiPrep, 1/16", male connector for ÄKTAdesign	28-4010-81	USD 69.29	Add
3	Fingertight Connector 1/16" Male for Tubing o.d. 1/16"	18-1112-55	USD 72.49	Add