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HiTrap SP HP

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  • Price:
    USD 142.75
  • Product code:
    17-1151-01
Complete Packsize:

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HiTrap SP HP, 5 x 1 ml

HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

  • 34 µm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Strong sulfopropyl (SP) cation exchanger. 
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as ÄKTA design.

The small particle size (34µm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

HiTrap SP HP, 5 x 1 ml

Pressure Max. [Over the Packed Bed During Operation] 3 bar [0.3 MPa] (42 psi)
Bed Dimensions 7 × 25 mm
Bed Volume 1 ml
Flow Rate < 4 ml/min1)
Storage Conditions 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
Average Particle Size 34 µm
Ligand Sulphopropyl
Ion Exchanger Type Strong cation exchanger
Pressure/Flow Specification 150 cm/h (at < 3 bar, 25°C, 10 cm bed height)
Storage Conditions 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
BioProcess Medium Yes
Ionic Capacity 0.15-0.20 mmol H+/ml 
pH Stability Cleaning 3-14
pH Stability Working Range 4-13
Exclusion Limit [Mr] [Globular Proteins] 4 x 106
Chemical Stability Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS1)
Charged Groups - SO3-
Matrix 6% cross-linked agarose
Particle Size 24 µm-44 µm
1)1 M NaOH and acetic acid should be used only for cleaning purposes.
Column
Column i.d. 7 mm
Material [Column Hardware] Polypropylene (PP)
Complete Packsize 1 ml

HiTrap SP HP, 5 x 1 ml



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