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HiTrap SP HP
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HiTrap SP HP
HiTrap SP HP are prepacked, ready-to-use SP Sepharose High Performance strong cation exchange columns for high-resolution, small-scale protein purification.
- 34 µm bead size for high-performance, high-resolution purifications.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump, and chromatography systems such as ÄKTA design.
- Strong sulfopropyl (SP) cation exchanger.
The small particle size (34µm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
HiTrap SP HP — Technical Specifications
|Bed Volume||1 ml|
|Bed Dimensions||7 × 25 mm|
|Flow Rate||< 4 ml/min1)|
|Flow rate||<4 ml/min2)|
|Storage Conditions||4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol|
|Pressure Max. [Over the Packed Bed During Operation]||3 bar [0.3 MPa] (42 psi)|
|1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|
|2)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|
|Average Particle Size||34 µm|
|Ion Exchanger Type||Strong cation exchanger|
|Matrix||6% cross-linked agarose|
|Particle Size||24 µm-44 µm|
|Ionic Capacity||0.15-0.20 mmol H+/ml|
|pH stability Working Range||4-13|
|pH Stability Working Range||4-13|
|pH Stability Cleaning||3-14|
|pH stability Cleaning||3-14|
|Pressure/Flow Specification||150 cm/h (at < 3 bar, 25°C, 10 cm bed height)|
|Exclusion Limit [Mr] [Globular Proteins]||4 x 106|
|Storage Conditions||4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate|
|Estimated Shelf Life from Manufacture Date||5 years|
|Chemical Stability||Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS1)|
|Charged Groups||- SO3-|
|Dynamic Capacity||55 mg ribonuclease/ml medium|
|Efficiency||> 12 000 m-1|
|1)1 M NaOH and acetic acid should be used only for cleaning purposes.|
|Complete Packsize||1 ml|
|Column i.d.||7 mm|
|Material [Column Hardware]||Polypropylene (PP)|