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HiTrap SP Sepharose FF

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  • Price:
    USD 134.32
  • Product code:
    17-5054-01
Complete Packsize:

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HiTrap SP FF

HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

HiTrap SP FF

Bed Volume 1 ml
Bed Dimensions 7 x 25 mm
Flow rate <4 ml/min1)
Storage Conditions 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
Pressure Max. [Over the Packed Bed During Operation] 3 bar [0.3 MPa] (42 psi)
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
BioProcess Media Yes
Ligand Sulphopropyl
Average Particle Size 90 µm
Ion Exchanger Type Strong cation exchanger
Matrix 6% cross-linked agarose
Particle Size 45 µm-165 µm
Ionic Capacity 0.18-0.25 mmol H+/ml medium
pH stability Working Range 4-13
pH stability Cleaning 3-14
Pressure/Flow Specification 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Exclusion Limit [Mr] [Globular Proteins] 4 x 106
Storage Conditions 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
Estimated Shelflife from Manufacture Date 5 years
Chemical Stability Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol1)
1)1 M NaOH and acetic acid should be used only for cleaning purposes.
Column
Complete Packsize 1 ml
Column i.d. 7 mm
Material [Column Hardware] Polypropylene (PP)
Freight Conditions ROOM TEMPERATURE
RoHS Compliance Exempt
Certificate of Analysis N
Min. Order Quantity 1

HiTrap SP FF

Animation
HiTrap: Connecting to a chromatography system (ÄKTA)
HiTrap: To use with a syringe
Protein purification strategy (Capture, Intermediate Purification, Polishing)
The principle of Ion Exchange Chromatography
Data file
IEX Sepharose Fast Flow labpacks and prepacked columns Summary
Handbook
Ion Exchange Chromatography & Chromatofocusing, Principles and Methods Summary
Purifying Challenging Proteins - Principles and Methods Summary
Strategies for protein purification Summary
Instruction/protocol
HiTrap DEAE FF, 1 ml and 5 ml, HiTrap CM FF, 1 ml and 5 ml, HiTrap Q FF, 1 ml and 5 ml, HiTrap SP FF, 1 ml and 5 ml, HiTrap ANX FF (high sub), 1 ml and 5 ml Summary
HiTrap IEX Selection Kit Summary
Magazine article
Redesigned HiTrap fittings offer easier connection to ÄKTAdesign chromatography systems (Life Science News 18, p19) Summary
Strategies for antibody purification
Material Safety Data Sheet
HiTrap SP FF 1 ml, 5 x 1 ml
Reference list
HiTrap SP HP and HiTrap SP FF Summary
Selection guide
Guide sheet ÄKTAFPLC Summary
Guide sheet ÄKTAprime plus Summary
Ion exchange columns and media - Selection guide Summary
Prepacked chromatography columns for ÄKTA™ design systems Summary
Protein and Peptide Purification: Technique selection guide Summary

HiTrap SP FF



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