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HiTrap SP Sepharose FF
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HiTrap SP FF
HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
HiTrap SP FF
|Bed Volume||1 ml|
|Bed Dimensions||7 x 25 mm|
|Flow rate||<4 ml/min1)|
|Storage Conditions||4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol|
|Pressure Max. [Over the Packed Bed During Operation]||3 bar [0.3 MPa] (42 psi)|
|1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|
|Average Particle Size||90 µm|
|Ion Exchanger Type||Strong cation exchanger|
|Matrix||6% cross-linked agarose|
|Particle Size||45 µm-165 µm|
|Ionic Capacity||0.18-0.25 mmol H+/ml medium|
|pH stability Working Range||4-13|
|pH stability Cleaning||3-14|
|Pressure/Flow Specification||400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm|
|Exclusion Limit [Mr] [Globular Proteins]||4 x 106|
|Storage Conditions||4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate|
|Estimated Shelflife from Manufacture Date||5 years|
|Chemical Stability||Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol1)|
|1)1 M NaOH and acetic acid should be used only for cleaning purposes.|
|Complete Packsize||1 ml|
|Column i.d.||7 mm|
|Material [Column Hardware]||Polypropylene (PP)|
|Freight Conditions||ROOM TEMPERATURE|
|Certificate of Analysis||N|
|Min. Order Quantity||1|