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GE Healthcare Life Sciences offers handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab.

Our handbooks provide theory, glossaries, workflows, protocols, recipes, and even troubleshooting guides to help make your experiments a success.

Download our handbooks below free of charge. If you would like to request printed hardcopies, please contact your local GE Healthcare representative.


NEW! Design of Experiments in Protein Production and Purification


An in-depth methodology handbook (PDF, 128 pages, 10 MB) to help you implement DoE techniques that will increase your output in protein expression and purification.

Learn More

Protein Sample Preparation Protein Purification Protein Analysis Nucleic Acids Cells

Protein Sample Preparation

Protein sample preparation is not only essential for optimal purification of your target protein, but crucial for analytical methods such as mass spectrometry, 2-D gel electrophoresis, 1-D SDS-PAGE, and molecular imaging.

 

Protein Sample Preparation

(9 MB, 123 pages)

 

Protein Purification

Although many proteins are easily purified today, the remaining challenges make it worthwhile to gain solid knowledge about protein purification so that appropriate methods can be selected and carried out in an optimal way.

 

Affinity Chromatography (4.7 MB, 159 pages)
Antibody Purification (5 MB, 162 pages)
Design of Experiments in Protein Production and Purification (10 MB, 128 pages)
Gel Filtration (Size Exclusion Chromatography) (12.1 MB, 123 pages)
GST Gene Fusion System (2.9 MB, 131 pages)
High Throughput Process Development (HTPD) with PreDictor Plates (4.4 MB, 60 pages)
Hydrophobic Interaction Chromatography (2.6 MB, 168 pages)
Ion Exchange Chromatography (2.2 MB, 187 pages)
Multimodal Chromatography (5.8 MB, 115 pages)
Purifying Challenging Proteins (2.5 MB, 107 pages)
Recombinant Protein Purification (5.5 MB, 308 pages)
Strategies for Protein Purification (12 MB, 167 pages)
ÄKTA Laboratory-Scale Chromatography Systems (9,8 MB, 92 pages)

 

Protein Analysis

The complexity of biological research requires sophisticated tools to visualize, quantitate, and identify proteins in complex samples and analyze their properties and interactions.

 

Biacore Assay Handbook

(1.3 MB, 78 pages)

Biacore Sensor Surface Handbook

(13.2 MB, 100 pages)

Molecular Imaging

(9.9 MB, 182 pages)

Spectrophotometry

(9.6 MB, 32 pages)

Western Blotting

(11 MB, 192 pages)

2-D Electrophoresis

(10.4 MB, 162 pages)

 

Nucleic Acids

RNA, genomic DNA, and plasmid DNA can be purified from a wide range of sample sources for a variety of downstream applications including blotting, sequencing, whole-genome analyses, gene expression, and expression analysis. To obtain the best possible results, care must be taken to purify nucleic acids in the appropriate quantity, quality, and purity for your downstream application and analysis. Highly sensitive biomolecular imagers and a range of fluorophores are available for qualitative and quantitative analyses.

 

Affinity Chromatography 

(4.7 MB, 159 pages)

Biacore Assay Handbook

(1.3 MB, 78 pages)

Biacore Sensor Surface Handbook

(13.2 MB, 100 pages)

Gel Filtration (Size Exclusion Chromatography)

(12.1 MB, 123 pages)

GST Gene Fusion System

(2.9 MB, 131 pages)

Molecular Imaging

(9.9 MB, 182 pages)

Nucleic Acid Sample Preparation for Downstream Analyses

(9.1 MB, 168 pages)

Spectrophotometry

(9.6 MB, 32 pages)

 

Cells

For decades, Ficoll and Percoll products have been used in density gradient centrifugation to isolate different types of cell populations from a variety of sample sources. Cell isolation and cell culture techniques are vital for studying cell structure, function and differentiation, as well as for the production of biologics. For anchorage-dependent cells, microcarriers can provide high-yield cell cultures by allowing cells to grow on the surface of the microcarrier beads.

 

Cell Separation Media (Density Gradient Centrifugation Media) (1.2 MB, 80 pages)
Isolation of Mononuclear Cells (223 KB, 20 pages)
Microcarrier Cell Culture (4.4 MB, 171 pages)