Proteins

Protein sample preparation is not only essential for optimal purification of your target protein, but crucial for analytical methods such as mass spectrometry, 2-D gel electrophoresis, 1-D SDS-PAGE, and molecular imaging. Although many proteins are easily purified today, the remaining challenges make it worthwhile to gain solid knowledge about protein purification so that appropriate methods can be selected and carried out in an optimal way.

Sample Preparation

Protein Sample Preparation (9 MB, 123 pages)

Protein Purification

Strategies for Protein Purification (12 MB, 167 pages)

Antibody Purification (5 MB, 162 pages)

Purifying Challenging Proteins (2.5 MB, 107 pages)

Recombinant Protein Purification (5.5 MB, 308 pages)

High Throughput Process Development (HTPD) with PreDictor Plates (4.4 MB, 60 pages)

Affinity Chromatography (4.7 MB, 159 pages)

Ion Exchange Chromatography (2.2 MB, 187 pages)

Gel Filtration (Size Exclusion Chromatography) (12.1 MB, 123 pages)

Hydrophobic Interaction Chromatography (2.6 MB, 168 pages)

ÄKTA Laboratory-Scale Chromatography Systems (9,8 MB, 92 pages) 

GST Gene Fusion System (2.9 MB, 131 pages)

Protein Analysis

2-D Electrophoresis (10.4 MB, 162 pages)

Western Blotting (11 MB, 192 pages)

Molecular Imaging (9.9 MB, 182 pages)

Spectrophotometry (9.6 MB, 32 pages)

Biacore Assay Handbook (1.3 MB, 78 pages)

Biacore Sensor Surface Handbook (13.2 MB, 100 pages)

MicroCal Calorimetry—Achieving High Quality Data (37.8 MB, 182 pages)

Nucleic Acids

RNA, genomic DNA, and plasmid DNA can be purified from a wide range of sample sources for a variety of downstream applications including blotting, sequencing, whole-genome analyses, gene expression, and expression analysis. To obtain the best possible results, care must be taken to purify nucleic acids in the appropriate quantity, quality, and purity for your downstream application and analysis. Highly sensitive biomolecular imagers and a range of fluorophores are available for qualitative and quantitative analyses.

Nucleic Acid Sample Preparation for Downstream Analyses (9.1 MB, 168 pages)

Affinity Chromatography (4.7 MB, 159 pages)

Gel Filtration (Size Exclusion Chromatography) (12.1 MB, 123 pages)

Molecular Imaging (9.9 MB, 182 pages)

Spectrophotometry (9.6 MB, 32 pages)

Biacore Assay Handbook (1.3 MB, 78 pages)

Biacore Sensor Surface Handbook (13.2 MB, 100 pages)

MicroCal Calorimetry—Achieving High Quality Data (37.8 MB, 182 pages)

GST Gene Fusion System (2.9 MB, 131 pages)