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Protein Purification Webinar Protein A Chromatography Webinar

How to Combine Protein Purification Techniques to Optimize Your Results

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Abstract

Significant improvements in a chromatography workflow can be achieved with small changes and proactive choices.

Before starting protein purification, it is important to define your requirements and objectives. These may include outlining the required purity, activity and the quantity of the target you want to achieve, ensuring process steps and handling are kept to a minimum and all critical impurities are removed.

This educational session is aimed to give guidance to protein purification protocol development for laboratory scale, and is designed for researchers working with either tagged or native proteins.

A key component of this Webinar is the introduction of a proven protein purification strategy, called CiPP (Capture, Intermediate purification, Polishing). It explains how to best combine the main separation techniques in order to obtain a powerful purification protocol delivering the pure and active protein you need for your work.

Insights into Recent Developments in Protein A Chromatography

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Abstract

Protein A-based  chromatography is the primary method used to purify monoclonal antibodies (MAbs). Despite much discussion about the high purchase costs, protein A medium (resin) still is a cost-efficient alternative to  most other purification techniques.

Over the past decade, there has been a significant development of protein A media, giving both higher capacity and improved stability to meet the changing requirements of the industry, and more improvements are to come.

This presentation will provide a brief background to the developments that have been made to improve cleanabilty and the overall capacity for MAbs. We will discuss ligand and base matrix (bead) designs, and provide insights into how these are modified by suppliers to optimize the medium to meet specific purification challenges. We will look at some of the limitations of protein A media when used in the industry, and how these limitations can be addressed through proper medium and process design.

Finally, we will provide some insights into the future plans of GE Healthcare’s Life Sciences business for protein A chromatography media and our thoughts on what will be needed for the next generation of products.

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Insights into Recent Developments in Protein A Chromatography

This presentation will provide a brief background to the developments that have been made to improve cleanabilty and the overall capacity for MAbs.

How to Combine Protein Purification Techniques to Optimize Your Results

This educational session is aimed to give guidance to protein purification protocol development for laboratory scale, and is designed for researchers working with either tagged or native proteins.

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