/Technology Insights/Chromatography/chromatography-465x262.jpg)
The term chromatography, which literally means “to write with color”, was first introduced in the early 1900s to describe its use in separating plant pigments. In chromatography, molecules are separated by dissolving a mixture in a mobile phase (e.g., buffer) and passing it through a stationary phase (e.g., chromatography beads).
By the second half of the century, several techniques quickly emerged using paper, gas, and liquid chromatography to separate a variety of molecules.
In 1959, Pharmacia (now GE Healthcare) led the way by introducing Sephadex, the world’s first gel filtration chromatography media (resin). Today, our range of chromatography systems and media cover all different types of liquid chromatography and all scales of purification from research to process-scale.
Types of matrices, types of elution, and protein purification strategies.
.png)
Separation based on ligand recognition, Immobilized metal ion affinity chromatography (IMAC)
_blur.png)
Separation based on isoelectric point
.png)
Also known as Gel Filtration (GF). Separation based on size.
.png)
Separation based on differences in surface hydrophobicity.
Separation based on charge
Separation based on a combination of parameters such as charge and hydrophobicity
Separation based on hydrophobicity under conditions where the chromatography medium is more hydrophobic than the mobile phase
