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Sephacryl Size Exclusion Media

Sephacryl High Resolution (HR) chromatography media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scales.

Sephacryl is composed of allyl dextran and N,N’-methylene bisacrylamide, cross-linked to form a strong and stable matrix, and may be a useful alternative to Superdex prep grade for applications that require a broader fractionation range. The high chemical stability of Sephacryl has led to its adaptation to industrial scale processes such as serum protein purification.

Sephacryl HR media are available in prepacked HiPrep Sephacryl HR gel filtration columns. These columns are fully compatible with chromatography systems in the ÄKTA design platform, adding automation and convenience to the excellent purification properties of Sephacryl.

Key Features

  • Excellent purification over a wide molecular weight range
  • High reproducibility due to high stability
  • High flow rates and recoveries
  • Well-suited to industrial scale use

 

Sephacryl media cover a wide fractionation range (from Mr 103 to >108). The product range includes media of six alternative porosities (related to dextran content), with each porosity giving a different selectivity profile.

Sephacryl S-100 HR for the separation of peptides and small proteins.

Sephacryl S-200 HR and Sephacryl S-300 HR for purifying antibodies, serum proteins, and mid-size proteins.

Sephacryl S-400 HR and Sephacryl S-500 HR for separating macromolecules such as complex polysaccharides.

Sephacryl S-1000 SF for purifying small particles such as plasmid DNA.

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