Choosing a country will allow you to access local content and enable you to shop in your local currency.
The product you are viewing is not available for online purchase.
Product to be discontinued and not replaced. Last order date is 30 June 2015
DeCyder 2-D Differential Analysis Software (DeCyder 2D) v7.2 is specifically designed for 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE)analysis and is a key element in the Ettan™ DIGE system. Unlike other 2-D electrophoresis methods, DIGE allows the option of reference to an internal standard for each spot, effectively eliminating gel-to-gel variation and delivering highly accurate, reproducible, and dependable quantitation. DeCyder 2D applies a gel comparison method that introduces zero statistical error, offering reliable data and analysis for 2-D DIGE experiments.
DeCyder 2-D v7.2 consists of several modules:
DeCyder 2D v7.2 includes functionalities not present in previous versions. An integrated image editor enables preparation of raw images (cropping, rotating, etc.) prior to analysis and also allows high-resolution images to be exported. The warping function improves matching accuracy and simplifies the evaluation of matching results. Improved gel image functionality includes gel overlay for match editing and additional spot editing functions. The "save as" feature allows data to be saved at any stage of the process
The DeCyder EDA module, standard in DeCyder 2D v7.2, offers support throughout the statistical analyses of 2-D DIGE data, with no requirement for statistical expertise. DeCyder EDA provides multivariate statistics tools for easy and fast data mining of 2-D DIGE data. The software enables the combined analysis of different datasets, aiding the biological interpretation of results by matching with data retrieved from public and local databases
DeCyder 7.2 includes a new functionality, available as an option, to normalize abundance values using proteins added as spikes (Spike Protein Normalization, SPN). This function complements the present model-based normalization. SPN is suitable for applications with samples that have few protein spots or samples where the majority of the proteins differ in abundance (or expression level) between sample groups. Prerequisite for the function is that one or several spike proteins are added to samples prior to CyDye labeling.
1. Alban, A. et al. A novel experimental design for comparative two-dimensional gel analysis: two-dimensional difference gel electrophoresis incorporating a pooled internal standard. Proteomics 3, 3644 (2003).
2. Yan, J. X. et al. Fluorescence 2-D Difference Gel Electrophoresis and mass spectrometry based proteomic analysis of Escherichia coli. Proteomics 2, 16821698 (2002).
3. Unlu, M. et al. Difference gel electrophoresis: a single method for detecting changes in protein extracts. Electrophoresis 18, 20712077 (1997).
4. Tonge, R. P. et al. Validation and development of fluorescence two-dimensional difference gel electrophoresis proteomics technology. Proteomics 1, 377396 (2001).
|Complete Packsize||1 Piece|
Experience the power of 2-D electrophoresis with 2-D DIGE
2-D electrophoresis is a standard technique for visualizing complex protein mixtures from biological samples. However, there are drawbacks since it is time consuming and data quality varies due to gel-to-gel variation. 2-D DIGE enables you to overcome the limitations of conventional 2-D electrophoresis. The ability to multiplex, and the use of an internal standard, allows you to use your time efficiently and extract the most information from every gel. Close
The full imaging spectrum
GE Healthcare Life Sciences offers a broad range of high-quality products covering 1-D and 2-D electrophoresis, Western blotting, and 2-D Difference Gel Electrophoresis (2-D DIGE). Common to all of these analytical workflows is image capture. Our portfolio of versatile and upgradeable imagers covers the entire range of protein and nucleic acid detection methods–from densitometry, through phosphorimaging to fluorescence and chemiluminescence–so that you can select the most appropriate analysis platform for your applications. Close
Imaging - Principles and Methods
The objective of "Imaging – Principles and Methods" is to provide readers with a theoretical overview and practical guide to molecular imaging and quantitation. Close
Frequently asked questions about how to order and set up elicense files for GE Healthcare Life Sciences e-Licensed software. Close
We've detected that you're using an unsupported browser.You may continue browsing, but for the best experience, please use Mozilla Firefox (v.30+), Google Chrome (v.35+) or Internet Explorer (v.9+).