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Electrophoresis and Isoelectric Focusing (IEF)

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We offer a wide range of instrumentation, precast gels, reagents, and accessories designed for the qualitative and quantitative analysis of biomolecules analyzed by electrophoresis. You can rely on the consistent performance and application support that has earned our electrophoresis systems and products tens of thousands of citations in scientific publications worldwide.

Nucleic acids or proteins can be resolved using an electric field applied to a gel matrix. In 1-D electrophoresis, the gel is typically made of agarose or crosslinked polymer of acrylamide (PAGE) with a porosity that depends on the weight and/or structure of the target. In general, smaller molecules migrate faster than larger molecules.
Sodium dodecyl sulfate (SDS) is commonly added to protein samples and buffers. This breaks up aggregates and confers a negative charge to all proteins, ensuring they migrate toward the positively charged anode. In 2-D gel electrophoresis, proteins are first resolved according to isoelectric point (pI) using isoelectric focusing (IEF), and then resolved in the second dimension using SDS-PAGE.