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Chemiluminescent Labeling and Detection Reagents

Since the early 1990s, we have continuously developed enhanced chemiluminescence detection systems, which are among the most widely used detection reagents for Western blotting applications today. GE Healthcare offers a variety of chemiluminescence detection reagents to cover a wide range of experimental needs.

Enhanced chemiluminescent (ECL) detection is the most commonly used detection method in Western blot analysis. ECL is based on antibodies conjugated to horseradish peroxidase (HRP). HRP catalyzes the oxidation of luminol in the presence of peroxide, generating emission of low intensity light at 428 nm. The signal intensity is dependent of the number of HRP molecules and is proportional to the amount of antibody bound to the target molecule.

View a side-by-side comparison of our Amersham ECL Western blotting reagents.



Amersham ECL start
Amersham ECL start Western Blotting Detection Reagent

Chemiluminescent detection of medium- and high-expression proteins, with a long lasting signal that provides a longer time window for detection.

Amersham ECL Western blotting detection reagent
Amersham ECL Western Blotting Detection Reagent

Reliable chemiluminescent detection of medium- and high expression proteins.

Amersham ECL Prime Western Blotting Detection Reagent
Amersham ECL Prime Western Blotting Detection Reagent

Highly sensitive chemiluminescent detection reagent with long lasting signal for accurate detection of minute changes in protein levels.

Amersham ECL Select Western Blotting Detection Reagent

Very sensitive chemiluminescent detection reagent with highly intense signal output, for detection of medium to very low protein levels.

Amersham ECL Western Blotting Reagent Pack

Contains blocking and HRP-conjugated secondary antibodies. ECL detection reagent sold separately.

Amersham ECL Western Blotting Detection Kit

Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.

Amersham ECL HRP Conjugated Antibodies

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.

Amersham ECL Glycoprotein Detection Module

Nonradioactive labeling of carbohydrate moieties on proteins and detection using streptavidin-horseradish peroxidase and ECL reagents.

Anti-GST HRP Conjugate

This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents.

Anti-His Antibody
Anti-His Antibody

Anti-His Antibody is an IgG₂ subclass of monoclonal antibody directed against six histidine residues, which are commonly used as an affinity tag for recombinant fusion proteins.

Anti-GST Antibody
Anti-GST Antibody

Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST, Because of its polyclonal nature, it can recognize more than one epitope on GST.

Biotinylated Antibodies

The antibodies are supplied with dilution guidelines for relevant applications.