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Genomic DNA Amplification

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Our illustra GenomiPhi product range uses Phi29 DNA polymerase to generate large amounts of DNA from small starting amounts of eukaryotic genomic DNA by isothermal multiple strand displacement amplification (MDA). MDA generates long fragments of high-quality DNA for applications such as next-generation sequencing (NGS), real-time PCR, SNP genotyping arrays, array comparative genomic hybridization (aCGH), DNA archiving, forensic analysis, hybridization, and cloning.

In the illustra GenomiPhi family are kits to meet the demands of your applications, from starting with extremely small amounts of DNA, down to a single cell, to applications where high yields are required. illustra Single Cell GenomiPhi is designed for the lysis and amplification of the whole genome of a single cell. illustra GenomiPhi kits offer the choice of standard or high (HY) output yield and the option to use either a standard liquid format or GE Healthcare’s proprietary Ready-To-Go (RTG) bead format. Liquid formats can be stored at -20°C or -80°C, while RTG formats are shipped and stored at room temperature.

Products

illustra Single Cell GenomiPhi DNA Amplification Kit
illustra Single Cell GenomiPhi DNA Amplification Kit

Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

illustra GenomiPhi HY DNA Amplification Kit
illustra GenomiPhi HY DNA Amplification Kits

Illustra GenomiPhi HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 50 μg yield from nanogram amounts of DNA sample.

illustra GenomiPhi V2 DNA Amplification Kit
illustra GenomiPhi V2 DNA Amplification Kits

Illustra GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample.

illustra Ready-To-Go GenomiPhi DNA Amplification Kits
illustra Ready-To-Go GenomiPhi HY DNA Amplification Kits

illustra Ready-To-Go GenomiPhi HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample.

illustra Ready-To-Go GenomiPhi DNA Amplification Kit
illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kits

illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 12 to 20 μg yield from nanogram amounts of DNA sample.