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Monitor UPC-900

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Monitor UPC-900

Monitor UPC-900 is a high precision on-line monitor offering the possibility to measure UV, conductivity, and pH using a single instrument.

  • Real chromatographic information at a glance.
  • Wide range of select, high specificity UV wavelengths using a mercury or zinc lamp.
  • Designed for all chromatographic techniques used in analysis and purification of biomolecules.
  • Flow cells fit tightly in series to minimize dead volume and band-broadening effects.
  • Three monitors in one unit: UV, pH, and conductivity.
  • Operates as an integral component of ÄKTAFPLC or as a standalone unit.

Monitor UPC-900 provides UV and temperature-compensated pH and conductivity parameters in a single display. Temperature is monitored and displayed and can be recorded if the monitor is used in ÄKTAdesign systems.

The mercury lamp is available with 254 or 280 nm filters for detecting proteins; the zinc lamp is used with the 214 nm filter for detecting peptides or for increased protein detection sensitivity. Additional filters are available for special applications within the range of 313 to 546 nm. The 2 mm UV flow cell and the pH and conductivity flow cells are designed to accommodate flow rates up to 100 ml/min; the 5 mm UV flow cell is optimized for flow rates to 20 ml/min.

The conductivity monitor features a broad dynamic range that accommodates the high salt concentrations used in hydrophobic interaction chromatography and the low conductivity of reversed phase or desalting separations. The pH monitor is essential for optimal results in affinity, ion exchange, and chromatofocusing separations.

Monitor UPC-900

Depth 250 mm
Conductivity 1 µS/cm-999.9 mS/cm
Weight 8.5 kg
Width 160 mm
Protection Class IP 20, IP 21 (Housing)
IP 44 (Flow cell)
Includes Hg optics, 254 and 280 nm filters, 2 mm UV flow cell, conductivity flow cell, signal cable and flow cell holder. (pH electrode and flow cell can be ordered separately if desired.)
pH Stability Short Term 1-14
Material Polyetheretherketone (PEEK)
Polytetrafluoroethylene (PTFE)
Titanium alloy (Ti alloy)
Response Time 3 s [0-95% of step]
<10 s [0-95% of step] (pH unit)
Cell Constant 50 cm-1 ± 20
Wetted Part No
Drift ±100 x 10–6 AU/h at 254 nm
0.1 pH unit deviation/10 h
Guidance Other available monitors
Monitor UVis-920
Monitor UV-900
Complete Packsize 1
Accuracy ± 0.1 pH unit, temperature compensated
± 3% or ± 15 µS/cm maximum, short-term
±1% within 0–100 ml/min
Operating Temperature 4°C-60°C
Relative Humidity 20%-95% (non condensing)
Operating Pressure Max. 50 bar (5 MPa) (725 psi)
Baseline Noise ± 0.5% Maximum of full scale
Voltage 100-120/220-240 VAC
Height 200 mm
pH Stability Long Term 1-13
Linearity (5 mm cell) <3% up to 2 AU at 254 nm
< 5% up to 1 AU at 280 nm

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