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Monitor UPC-900 is a high precision on-line monitor offering the possibility to measure UV, conductivity, and pH using a single instrument.
Monitor UPC-900 provides UV and temperature-compensated pH and conductivity parameters in a single display. Temperature is monitored and displayed and can be recorded if the monitor is used in ÄKTAdesign systems.
The mercury lamp is available with 254 or 280 nm filters for detecting proteins; the zinc lamp is used with the 214 nm filter for detecting peptides or for increased protein detection sensitivity. Additional filters are available for special applications within the range of 313 to 546 nm. The 2 mm UV flow cell and the pH and conductivity flow cells are designed to accommodate flow rates up to 100 ml/min; the 5 mm UV flow cell is optimized for flow rates to 20 ml/min.
The conductivity monitor features a broad dynamic range that accommodates the high salt concentrations used in hydrophobic interaction chromatography and the low conductivity of reversed phase or desalting separations. The pH monitor is essential for optimal results in affinity, ion exchange, and chromatofocusing separations.
|Includes||Hg optics, 254 and 280 nm filters, 2 mm UV flow cell, conductivity flow cell, signal cable and flow cell holder. (pH electrode and flow cell can be ordered separately if desired.)|
± 0.1 pH unit, temperature compensated
± 3% or ± 15 µS/cm maximum, short-term
±1% within 0–100 ml/min
|Operating Pressure Max.||50 bar (5 MPa) (725 psi)|
|Conductivity||1 µS/cm-999.9 mS/cm|
|Relative Humidity||20%-95% (non condensing)|
|pH Stability Long Term||1-13|
|pH Stability Short Term||1-14|
(5 mm cell) <3% up to 2 AU at 254 nm
< 5% up to 1 AU at 280 nm
|Baseline Noise||± 0.5% Maximum of full scale|
±100 x 10–6 AU/h at 254 nm
0.1 pH unit deviation/10 h
|Cell Constant||50 cm-1 ± 20|
3 s [0-95% of step]
<10 s [0-95% of step] (pH unit)
IP 20, IP 21 (Housing)
IP 44 (Flow cell)
Titanium alloy (Ti alloy)
Other available monitors
Monitor UPC-900 is a high precision on-line monitor offering the unique possibility to measure UV, conductivity and pH using a single instrument. The monitor is used as a stand-alone instrument, or as a component in ÄKTA™ FPLC™ , to record accurate information during any liquid chromatographic separation. Temperature is monitored and displayed, but can also be recorded if Monitor UPC-900 is used in ÄKTAFPLC. ÄKTAFPLC is one of a series of ÄKTAdesign high performance systems for purification and analysis of biomolecules. Close
UV lamp exchange
This instruction is valid for the Hg and Zn lamps. Close
Flow cell, Installation instructions
This instruction is a complement to Monitor UPC-900 User Manual (no. 18-1125-55). The instruction describes how a conductivity cell and an optical unit should be connected to the module, when the module is used as a stand-alone unit. It also describes the general installation of the module. Close
Monitor UPC-900 is a high precision on-line monitor for the combined measurement of UV absorption, pH and conductivity in liquid chromatography. The UPC-900 features: Fixed wavelengths of 254, 280 nm (Hg lamp), or 214 nm (Zn lamp). Other wavelengths of 313, 365, 405, 436 and 546 nm through filter change. Two alternative flow cells with pathlengths of 2 mm and 5 mm. Fast response High accuracy and reproducibility Flow cells with low dead volume Accurate and reliable monitoring through self-test and self-calibration Flow cells can be connected close together, minimising band broadening and time delay between detectors. Close
Monitor UPC-900 Operating Instructions Original instructions
The Operating Instructions provide you with the instructions needed to handle Monitor UPC-900 in a safe way. Close
Monitor UPC-900, Short Instructions
Concise, illustrated, instructions for the usage of the Monitor UPC-900. Close
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