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PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
Each product pack includes 30 columns, a buffer tray, a column stand, and 4 adapters for centrifugation.
The product instructions include two protocols. The gravity protocol allows simple processing of samples using one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
The following publications demonstrate the utility of PD-10 columns:
A LabMate buffer reservoir can be used for one-step application of the column wash and equilibration buffer using the gravity flow protocol. It is not included with this product but can be purchased separately.
GE Healthcare’s Life Sciences solutions for desalting include a wide range of columns containing Sephadex G-25 resin. Options are available to accommodate sample volumes from 100 µL to 15 mL and use with manual methods or a chromatography system. Let GE help you find the right desalting product.
|Bed Dimensions||14.5 × 50 mm|
1 × Column stand
4 × PD-10 spin adaptor
1 × Buffer tray
30 × Bottom sleeve
Instructions are supplied with the columns
|Bed Volume||8.3 ml|
|Sample Volume||2.5 ml|
|Complete Packsize||30 Columns|
|Storage Conditions||4 to 30°C, 0.15% Kathon CG/ICP Biocide|
|Storage||4 to 30°C, 0.15% Kathon CG/ICP Biocide|
Disposable columns for clean-up of proteins/oligosaccharides.
The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow.
PD- 10 Buffer reservoir has to be ordered separately
For smaller scale please use PD MidiTrap G-25 (1.0 mL), PD MiniTrap G-25 (0.5 mL), PD SpinTrap G-25 (130 µL) and PD MultiTrap G-25 (130 µL) in parallel
|Particle size distribution, wet||38–235 µm|
|Partical Size Dry||>50 µm|
|pH stability, operational||2–131)|
|pH stability, CIP||2–132)|
|Pressure/Flow Specification||>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.|
|Fractionation range [Mr], Globular proteins||1 × 103–5 × 103|
|Fractionation [Mr] Dextrans||1 x 102–1 x 102|
|Exclusion Limit [Mr] [Globular Proteins]||> 5 × 103|
|Exclusion Limit||Mr 5000|
|Storage||4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH|
|Chemical Stability||Stable in commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile|
|Certificate of Analysis||Yes|
|1)pH range where resin can be operated without significant change in function.|
|2)pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function.|
|Column i.d.||15 mm|
|Chemical Stability||Stable in all commonly used buffer systems|
|Material [Column Hardware]||Polypropylene (PP)|
|Material [Bed Support]||Polyethylene (PE)|
The Principle of Gel Filtration
Rapid and efficient purification and refolding of a (His)6-tagged recombinant protein produced in e.coli as inclusion bodies