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HisTrap FF

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HisTrap FF

HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • High binding capacity, approx. 40 mg/ml medium.
  • Negligible leakage of Ni2+.
  • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications

The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

HisTrap FF

Bed Volume 1 ml
Pressure Max. [Over the Packed Bed During Operation] 5 bar [0.5 MPa] (70 psi)
Flow Rate < 4 ml/min1)
Order Information Use 20-40 mM imidazole in sample and binding buffers for highest purity.
Storage Conditions 4 to 30°C, 20% Ethanol
Bed Dimensions 7 × 25 mm
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Pressure/Flow Specification Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
pH Stability Working Range 3-121)
BioProcess Medium Yes
Storage Conditions 4 to 30°C, 20% Ethanol
pH Stability Cleaning 2-142)
Binding Capacity/ml Chromatography Medium Approx. 40 mg histidine-tagged protein
Metal Ion Capacity ~15 µmol Ni2₊/ml medium
Matrix 6% cross-linked agarose
Particle Size 45 µm-165 µm
Chemical Stability Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3)
Average Particle Size 90 µm
1)Ni²⁺-stripped medium.
2)Ni²⁺-stripped medium.
3)Ni²⁺-stripped medium.
Material [Column Hardware] Polypropylene (PP)
Column i.d. 7 mm
Complete Packsize 1 ml


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HisTrap FF

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