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Handbooks

GE Healthcare Life Sciences offers principles and methodology handbooks covering a wide range of techniques and applications including sample preparation, protein purification, electrophoresis, blotting, and cell culture. Whether you’re a beginner or an expert, our handbooks are a great reference for practical tips and in-depth information about common methodologies used in the lab.

New Affinity Chromatography Handbook out now!

Affinity Chromatography Handbook, Vols. 1 to 3 present the most effective and most frequently used strategies for sample preparation and purification of proteins using affinity chromatography in the laboratory. The three volumes cover purification of: 1. antibodies/antibody fragments; 2. tagged proteins; and 3. other specific groups of biomolecules. The blend of general guidance and specific examples will be of enormous value to both novice and expert in developing a successful affinity purification strategy for a wide range of biomolecules.

Affinity Chromatography Vol. 1: Antibodies

Affinity Chromatography Vol. 2: Tagged Proteins

Affinity Chromatography Vol. 3: Specific Groups of Biomolecules

Protein Purification Handbooks Protein Sample Preparation Protein Analysis Nucleic Acids Cells

Protein Purification

Although many proteins are easily purified today, the remaining challenges make it worthwhile to gain solid knowledge about protein purification so that appropriate methods can be selected and carried out in an optimal way.

Antibody Purification (Affinity chromatography, Vol.1) (3.13 MB, 173 pages)

Cross Flow Filtration Method Handbook (1.04 MB, 82 pages)

Design of Experiments in Protein Production and Purification (10 MB, 128 pages)

GST Gene Fusion System (1.7 MB, 132 pages)

High Throughput Process Development (HTPD) with PreDictor Plates (4.3 MB, 60 pages)

Hydrophobic Interaction Chromatography (2.5 MB, 168 pages)

Ion Exchange Chromatography (9.06 MB, 170 pages)

Multimodal Chromatography (5.5 MB, 115 pages)

Purification of specific groups of biomolecules (Affinity chromatography, Vol.3) (3.40 MB, 153 pages)

Purifying Challenging Proteins (2.4 MB, 107 pages)

Size Exclusion Chromatography (3.4 MB, 139 pages)

Strategies for Protein Purification (12 MB, 167 pages)

Tagged Protein Purification (Affinity chromatography, Vol.2) (6.09 MB, 294 pages)

ÄKTA Laboratory-Scale Chromatography Systems (6,5 MB, 106 pages)

Protein Sample Preparation

Protein sample preparation is not only essential for optimal purification of your target protein, but crucial for analytical methods such as mass spectrometry, 2-D gel electrophoresis, 1-D SDS-PAGE, and molecular imaging.

Cross Flow Filtration Method Handbook (1.04 MB, 82 pages)

Protein Sample Preparation (7 MB, 136 pages)

Protein Analysis

The complexity of biological research requires sophisticated tools to visualize, quantitate, and identify proteins in complex samples and analyze their properties and interactions.

Biacore Assay Handbook (1.3 MB, 78 pages)

Biacore Sensor Surface Handbook (13.2 MB, 100 pages)

Molecular Imaging (9.6 MB, 182 pages)

Spectrophotometry (1.3 MB, 32 pages)

Western Blotting (9.9 MB, 176 pages)

2-D Electrophoresis (8.9 MB, 162 pages)

Nucleic Acids

RNA, genomic DNA, and plasmid DNA can be purified from a wide range of sample sources for a variety of downstream applications including blotting, sequencing, whole-genome analyses, gene expression, and expression analysis. To obtain the best possible results, care must be taken to purify nucleic acids in the appropriate quantity, quality, and purity for your downstream application and analysis. Highly sensitive biomolecular imagers and a range of fluorophores are available for qualitative and quantitative analyses.

Affinity Chromatography (4.7 MB, 159 pages)

Biacore Assay Handbook (1.3 MB, 78 pages)

Biacore Sensor Surface Handbook (13.2 MB, 100 pages)

Cross Flow Filtration Method Handbook (1.04 MB, 82 pages)

GST Gene Fusion System (1.7 MB, 131 pages)

Molecular Imaging (9.6 MB, 182 pages)

Nucleic Acid Sample Preparation for Downstream Analyses (9.1 MB, 168 pages)

Size Exclusion Chromatography (3.4 MB, 139 pages)

Spectrophotometry (1.3 MB, 32 pages)

Cells

For decades, Ficoll and Percoll products have been used in density gradient centrifugation to isolate different types of cell populations from a variety of sample sources. Cell isolation and cell culture techniques are vital for studying cell structure, function and differentiation, as well as for the production of biologics. For anchorage-dependent cells, microcarriers can provide high-yield cell cultures by allowing cells to grow on the surface of the microcarrier beads.

Cell Separation Media (Density Gradient Centrifugation Media) (1.2 MB, 80 pages)

Cross Flow Filtration Method Handbook (1.04 MB, 82 pages)

Isolation of Mononuclear Cells (223 KB, 20 pages)

Microcarrier Cell Culture (4.4 MB, 171 pages)