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HiTrap SP XL

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  • Price:
    USD 300.00
  • Product code:
    17-5161-01
Complete Packsize:

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HiTrap SP XL

HiTrap SP XL are strong cation exchangers prepacked with SP Sepharose XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA design.
  • High binding capacities with high flow rates.

Q Sepharose XL and SP Sepharose XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

HiTrap SP XL

Flow Rate < 20 ml/min1)
Pressure Max. [Over the Packed Bed During Operation] 5 bar [0.5 MPa] (70 psi)
Storage Conditions 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
Bed Dimensions 16 × 25 mm
Bed Volume 5 ml
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
Pressure/Flow Specification 300-500 cm/h
Storage Conditions 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
Ligand Sulphopropyl
Average Particle Size 90 μm
pH Stability Cleaning 3-14
Matrix 6% cross-linked agarose with dextran surface extenders
pH Stability Working Range 4-13
Ionic Capacity 0.18-0.25 mmol H+/ml medium
Particle Size 45 μm-165 μm
Chemical Stability All commonly used buffers, nonionic detergents, 1 M NaOH, 6 M guanidine hydrochloride
BioProcess Medium Yes
Ion Exchanger Type Strong cation exchanger
Column
Column i.d. 16 mm
Material [Column Hardware] Polypropylene (PP)
Complete Packsize 5 ml

HiTrap SP XL



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