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Sephadex LH-20

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Sephadex LH-20, 100 g

Sephadex LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species

  • Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
  • Designed for use in polar organic solvents and aqueous solvent mixtures
  • Excellent batch-to-batch reproducibility
  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
  • Sephadex LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
  • Useful for both analytical and industrial scales for the preparation of closely related molecular species
  • Sephadex LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.

Sephadex LH-20 is prepared by hydroxypropylation of Sephadex G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.

Sephadex LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility

Sephadex LH-20, 100 g

Matrix Hydroxypropylated, cross-linked dextran
Exclusion Limit Peptides 4 x 103
Storage Conditions 4 to 30°C
Exclusion Limit Small Organics 5 x 103
Estimated Shelf Life from Manufacture Date 8 years
Particle Size in Methanol Min. 27 µm
Chemical Stability Stable in most aqueous and organic eluent systems.
Autoclavable 20 min at 121°C
Particle Size in Methanol Max. 163 µm
Flow Velocity < 60 cm/hour
Partical Size Dry 18 µm-111 µm
Fractionation [Mr] Globular Proteins 4 × 103 (Exclusion limit will depend on the solvent) – 5 × 103 (Exclusion limit will depend on the solvent)
pH Stability Cleaning-in-Place (CIP) 2-13
Avoid Using Not stable below pH 2 nor to strong oxidising agents
Sample Loading Volume Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]
Pressure/Flow Specification 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
pH Stability Working Range 2-13


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