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Butyl-S Sepharose 6 Fast Flow

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Butyl-S Sepharose 6 Fast Flow, 200 ml

Butyl-S Sepharose 6 Fast Flow is the least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities

  • The least hydrophobic medium in the well proven Sepharose Fast Flow HIC range
  • Binds and elutes relatively strong hydrophobic molecules at comparatively low salt concentrations
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
  • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
  • Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals

Butyl-S Sepharose 6 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

Butyl-S Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose 4 Fast Flow and other HIC Sepharose Fast Flow products.

This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations. Butyl‑S Sepharose 6 Fast Flow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

Butyl-S Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Butyl-S Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Butyl-S Sepharose 6 Fast Flow, 200 ml

Matrix 6% cross-linked agarose
pH Stability Working Range 3-13
Storage Conditions 4 to 30°C, 20% Ethanol
pH Stability Cleaning-in-Place (CIP) 2-14
Certificate of Analysis Yes
Pressure/Flow Specification Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
BioProcess Medium Yes
Particle Size 45 µm-165 µm
Ligand Butyl-S
Chemical Stability Stable in commonly used aqueous buffers: 1 mM HCl, 1 M NaOH, 30% isopropanol, 50% ethylene glycol, 70% ethanol, 6 M guanidinehydrochloride, 8 M urea
Average Particle Size 90 µm
Degree of Substitution Approx 10 µmol Butyl-S/ml medium

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