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HiTrap Q Sepharose FF

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HiTrap Q FF

HiTrap Q FF is prepacked with Q Sepharose Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • Predictable scale-up
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

HiTrap Q FF

Storage Conditions 4 to 30°C, 20% Ethanol
Flow Rate < 4 ml/min1)
Pressure Max. [Over the Packed Bed During Operation] 5 bar [0.5 MPa] (70 psi)
Bed Dimensions 7 × 25 mm
Bed Volume 1 ml
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Storage Conditions 4 to 30°C, 20% Ethanol
Ionic Capacity 0.18-0.24 mmol Cl-/ml medium
pH Stability Cleaning 2-14
BioProcess Medium Yes
pH Stability Working Range 2-12
Pressure/Flow Specification 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Particle Size 45 µm-165 µm
Matrix 6% cross-linked agarose
Exclusion Limit [Mr] [Globular Proteins] 4 x 106
Ion Exchanger Type Strong anion exchanger
Ligand Quaternary amine
Average Particle Size 90 µm
Chemical Stability Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol1)
1)1 M NaOH and acetic acid should be used only for cleaning purposes.
Complete Packsize 1 ml
Column i.d. 7 mm
Material [Column Hardware] Polypropylene (PP)

HiTrap Q FF

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