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HiTrap SP Sepharose FF

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HiTrap SP FF

HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

  • High flow rates and good scale-up potential.
  • The industry standard for ion exchange chromatography.
  • Predictable scale-up
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

HiTrap SP FF

Pressure Max. [Over the Packed Bed During Operation] 5 bar [0.5 MPa] (70 psi)
Bed Volume 1 ml
Storage Conditions 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
Flow Rate < 4 ml/min1)
Bed Dimensions 7 × 25 mm
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
Particle Size 45 µm-165 µm
Matrix 6% cross-linked agarose
Ion Exchanger Type Strong cation exchanger
Storage Conditions 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
Ligand Sulphopropyl
Pressure/Flow Specification 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Ionic Capacity 0.18-0.25 mmol H+/ml medium
Exclusion Limit [Mr] [Globular Proteins] 4 x 106
BioProcess Medium Yes
pH Stability Working Range 4-13
pH Stability Cleaning 3-14
Chemical Stability Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol1)
Average Particle Size 90 µm
1)1 M NaOH and acetic acid should be used only for cleaning purposes.
Column
Complete Packsize 1 ml
Column i.d. 7 mm
Material [Column Hardware] Polypropylene (PP)

HiTrap SP FF



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