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Ni Sepharose 6 Fast Flow

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Ni Sepharose 6 Fast Flow, 1 l

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

  • Designed for scaling up purification of histidine-tagged proteins
  • High protein binding capacity and minimal leakage of Ni2₊ ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.

Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2₊ leakage while providing high capacity.

Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Ni Sepharose 6 Fast Flow, 1 l

Storage Conditions 4 to 30°C, 20% Ethanol
Particle Size 45 µm-165 µm
Pressure/Flow Specification Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Average Particle Size 90 µm
Matrix 6% cross-linked agarose
Metal Ion Capacity ~15 µmol Ni2₊/ml medium
Chemical Stability Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea1)
pH Stability Cleaning-in-Place (CIP) 2-142)
pH Stability Working Range 3-123)
Binding Capacity/ml Chromatography Medium Approx. 40 mg histidine-tagged protein
BioProcess Medium Yes
1)Ni²⁺-stripped medium.
2)Ni²⁺-stripped medium.
3)Ni²⁺-stripped medium.


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