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Capto adhere

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Capto adhere, 1 l

Capto adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process

  • Two-step chromatographic process with protein A affinity, saves time and cuts operating costs.
  • Multimodal anion exchanger for post-protein A purification of monoclonal antibodies.
  • Wide operational window of pH and conductivity.
  • High capacity and productivity in flow-through mode
  • Removes contaminants to formulation levels.

Capto adhere is a multimodal BioProcess medium designed for post-protein A purification of monoclonal antibodies (MAbs) at process scale. It is composed of a rigid, high-flow agarose matrix and a novel multimodal ligand. The strong multimodal ligand gives a different selectivity compared to traditional ion exchangers. Capto adhere can remove key contaminants such as DNA, host cell proteins (HCP), leached protein A, dimers and larger aggregates, and viruses in a single step. Together with a Protein A capture step, like MabSelect SuRe, Capto adhere allows the design of a two-step chromatographic process for Mabs.

For optimal performance of Capto adhere, screening/optimization of loading conditions is recommended, preferably using modern high-throughput process development tools, such as PreDictor 96-well filter plates.

Capto adhere is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Capto adhere meets industrial demands with security of supply and comprehensive technical and regulatory support.

Capto adhere, 1 l

Matrix Highly cross-linked agarose
Ionic Capacity 0.09-0.12 mmol Cl-/ml medium
Pressure/Flow Specification 300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height
Estimated Shelf Life from Manufacture Date 5 years
Ligand N-benzyl-n-methyl ethanolamine
Average Particle Size 75 µm (d50V)1)
pH Stability Working Range 3-122)
BioProcess Medium Yes
pH Stability Cleaning-in-Place (CIP) 2-143)
Ion Exchanger Type Multimodal strong anion exchanger
Storage Conditions 4 to 30°C, 20% Ethanol
Chemical Stability All commonly used aqueous buffers, 1 M acetic acid, 1 M sodium hydroxide4)
Certificate of Analysis Yes
1)d₅₀v is the median particle size of the cumulative volume distribution.
2)pH interval where the medium can be operated without significant change in function.
3)pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.
4)No significant change in ionic binding capacity and carbon content after one week storage in 1 M NaOH at 40°C.

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