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illustra MicroSpin G-25 Columns

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illustra MicroSpin G-25 Columns

MicroSpin G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

  • Tested in nickase, single and double-stranded exonuclease and RNase assays.
  • Excellent for rapid purification of newly synthesized oligonucleotides > 10-mers in 100 to 150 µl of deprotection solution using spin-column chromatography.
  • Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µl.
  • For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 µl using spin-column chromatography.
  • Convenient: Prepacked with Sephadex G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
  • Ready to use: Requires less than 4 min from sample application to collection of purified product.

MicroSpin G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.

illustra MicroSpin G-25 Columns

Complete Packsize 50 Columns
Sample Volume < 150 µl
Storage Conditions 20 to 25°C, Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative
Fractionation Range [Mr] DNA Exclusion Limit 10
pH Stability Working Range 2-13
Storage Conditions 0.01 M NaOH
Fractionation [Mr] Globular Proteins 1000 – 5000
Fractionation [Mr] Dextrans 100-5000
Partical Size Dry 20 µm-80 µm

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