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HiPrep Q FF

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HiPrep Q FF 16/10

HiPrep Q FF 16/10 is a strong anion exchanger prepacked with Q Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

  • Compatible with single-pump configurations and chromatography systems such as ÄKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • HiPrep columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • The industry standard for ion exchange chromatography.
  • Good flow rates and high capacity.

HiPrep Q FF are prepacked, ready to use columns for ion exchange chromatography. They provide fast, preparative separations of proteins and other biomolecules.

HiPrep Q FF 16/10

Flow Rate 2 ml/min - 10 ml/min1)
Bed Dimensions 16 × 100 mm
Storage Conditions 4 to 30°C, 20% Ethanol
Pressure Max. [Over the Packed Bed During Operation] 1.5 bar [0.15 MPa] (22 psi)
Bed Volume 20 ml
Complete Packsize 20 ml
Flow Velocity < 300 cm/h
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
BioProcess Medium Yes
pH Stability Working Range 2-12
pH Stability Cleaning 2-14
Ionic Capacity 0.18-0.24 mmol Cl-/ml medium
Chemical Stability Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol1)
Ion Exchanger Type Strong anion exchanger
Exclusion Limit [Mr] [Globular Proteins] 4 x 106
Particle Size 45 µm-165 µm
Matrix 6% cross-linked agarose
Storage Conditions 4 to 30°C, 20% Ethanol
Pressure/Flow Specification 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
Average Particle Size 90 µm
Ligand Quaternary amine
1)1 M NaOH and acetic acid should be used only for cleaning purposes.
Column
Material [Column Tube] Polypropylene (PP)
Material [Column Hardware] Polypropylene (PP)
Column i.d. 16 mm
Material [Bed Support] Polymethyl-Methacrylate (PMMA)
Bed Support Filter

HiPrep Q FF 16/10



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