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Typhoon FLA 9000


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Typhoon FLA 9000

Typhoon FLA 9000 is a versatile laser scanner for biomolecular imaging applications, including sensitive and quantitative measurement of radioisotopic labels,chemifluorescent Western blots, 2-D DIGE, multiplex fluorescence (visible and near infrared excitation) and colorimetric stains (e.g., Coomassie blue and silver stained gels).

  • High performance, flexible, and sensitive imaging for filmless autoradiography, fluorescence, chemifluorescence, 2-D DIGE and gel documentation
  • Large 40 x 46 cm scan area for simultaneous imaging of up to two DIGE Gels or sixteen 10 x 8 cm blots facilitates comparisons, reducing workload and waiting time
  • Use multiple imaging modes at any time with no system modifications, reducing effort
  • 16-bit images at up to 10 μm pixel resolution give precise quantitation in gels, blots, tissue sections and arrays
  • Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 32P, 33P, 39S, and other sources of ionizing radiation
  • Two- and three-color fluorescence scanning allows multiplex imaging (e.g., ECL Plex and 2-D DIGE), ensuring good quantitation. Flexible system can be adapted with stages, detectors, filters, and lasers to optimize performance in new applications. Optional near infrared excitation for imaging IRDye and other infrared excited dyes

Typhoon FLA 9000 is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules.

The system provides several imaging modes at any time, including fluorescence and chemifluorescence, filmless autoradiography, and digitization of colorimetrically-stained samples (e.g., Coomassie blue and silver stain). It is also adequate for quantitative chemiluminescent imaging that does not require maximum sensitivity.

The system is optimized for 2-D DIGE imaging for differential protein expression studies, ECL Plus chemifluorescent imaging for quantitative protein detection by Western blotting, and multifluorescent ECL Plex imaging for precise quantitation of two or more proteins in the same blot.

The system accommodates four automated filters. Filters are easily accessed and exchanged without tools for attaining optimal imaging conditions.

Stages give the optimal imaging conditions for a range of samples. Samples include agarose and polyacrylamide gels, membranes, DIGE gels, radioisotope-labeled samples, as well as microplates and glass slides with the TP plug-in. The system can simultaneously scan two DIGE gels, each measuring up to 20 x 25 cm. The stages are easily removed from the system for cleaning.

A fast 1000 μm prescan function gives a rapid overview of the sample. The system provides a linear signal response over five orders of magnitude, providing a suitable basis for precise quantitation.

Lasers can be exchanged in the field to accommodate new applications and fluorophores.

Typhoon FLA 9000

Complete Packsize 1 Piece
Detection Modes Fluorescence, chemifluorescence, filmless autoradiography, chemiluminescence and digitization
Radioisotopes 3H, 14C, 32P, 33P, 39S and 125I
Dynamic Range Five orders of magnitude
Bit Depth 16-bit
Scanning Area 40 x 46 cm
Pixel Size 10, 25, 50, 100, 200 and prescan 1000 μm, selectable
Standard Filters IP (Phosphorimaging), LPB (510LP), LPG (575LP), LPR (665LP), BPB1 (530DF20) and BPG1 (570DF20)
Height 400 mm
Depth 800 mm
Width 900 mm
Weight 97 kg
Frequency 50/60 Hz
Operating Temperature 15°C-30°C
Operating Humidity 0.2-0.7
Voltage 100-200 VAC ± 10 %
Power Consumption Approx. 0.3 kVA
Storage Conditions 10 ROOM TEMPERATURE
Freight Conditions ROOM TEMPERATURE
Min. Order Quantity 1
Certificate of Analysis N
RoHS Compliance Yes

Typhoon FLA 9000

Application note
A comparative evaluation of quantitative imaging of DNA and protein using stains and labels Summary
Biacore biosensor assays for quantitation of influenza virus and HCP Summary
Characterizing host cell protein patterns with 2-D DIGE improves process understanding Summary
Data file
PM fact sheet Scanners
Typhoon™ FLA 9000 biomolecular imager Summary
Handbook
2-D Electrophoresis, Principles and Methods Summary
Western Blotting - Principles and Methods Summary
Magazine
Discovery Matters issue 11 Summary
Magazine article
Screening of extraction buffers for 2-D DIGE analysis of plant proteins - article from Discovery Matters 13, May 2011 Summary
Synchronized solutions for 2-D electrophoresis and 2-D DIGE (from Discovery Matters 11, April 2010) Summary
User manual
Typhoon FLA 9000 Getting Started Summary
Typhoon FLA 9000 Quick start guide Summary
Typhoon FLA 9000 User Manual Summary

Typhoon FLA 9000



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