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HiLoad Superdex 200 PG

Column i.d. 16 mm
Bed Height 600 mm

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HiLoad 16/600 Superdex 200 pg

HiLoad 16/600 Superdex 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • High resolution with short run times and good recovery.
  • 1/16” fittings for convenient connections to ÄKTA design systems.
  • High capacity at high flow rates.

Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex 30 pg), Mr 3 000 to 70 000 (Superdex 75 pg), and Mr 10 000 600 000 (Superdex 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

HiLoad 16/600 Superdex 200 pg

Includes Dismantling tool, support screen, net ring (10 μm), O-ring, stop plugs, and instructions.
Complete Packsize 1 × 120 mL
Bed Dimensions 16 × 600 mm
Bed Volume 120 ml
Order Information HiLoad columns are prepacked XK columns.
Flow Rate < 1.0 ml/min
Sample Volume 5 ml
Theoretical Plates > 13000 m-1
Storage Conditions 4 to 30°C, 20% Ethanol
Pressure Max. [Over the Packed Bed During Operation] 3 bar [0.3 MPa] (42 psi)
Flow Velocity 10 cm/h - 50 cm/h1)
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Media
pH Stability Working Range 3-12
Particle Size 24-44 µm
pH Stability Cleaning 1-14
Matrix Composite of cross-linked agarose and dextran
BioProcess Medium Yes
Storage Conditions 4 to 30°C, 20% Ethanol
Fractionation [Mr] Dextrans 1 × 103-1 × 105
Fractionation [Mr] Globular Proteins 1 × 104 – 6 × 105
Average Particle Size 34 µm
Chemical Stability All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
Column
Complete Packsize 1 Piece
Material [Bed Support] Polypropylene (PP) (reinforced glass fiber), Polyamide (PA)
Bed Support Net
Inlet i.d. 0.8 mm (0.031 in)
Column i.d. 16 mm
Outlet i.d. 0.8 mm (0.031 in)
Material [Column Hardware] Borosilicate Glass, Polyamide, Fluororubber, Ethylene Propylene Diene (EPDM), ETFE, Superpolyoxymethylene
Porosity 10 μm
Material [Column Tube] Borosilicate Glass
Chemical Stability Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

HiLoad 16/600 Superdex 200 pg



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