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Processor Plus is a novel fluid delivery system that automates acrylamide gel staining and western blot processing. Changing from one application to the other is achieved by a fast, simple switch of a tray.
Processor Plus is a novel fluid delivery system that automates acrylamide gel staining and Western blot processing. Changing from one application to the other is achieved by a fast, simple switch of a tray. Select a programmed protocol and push start. Processor Plus will fill and empty the tray with the correct solution at the right time.
The blot-processing capabilities of Processor Plus include trays for four mini- (up to 9 × 9.5 cm) or two standard- (up to 16 × 16 cm) sized blots at one time. Five preprogrammed and five user-defined blot protocols are incorporated for flexibility, and microprocessor precision ensures consistent results with low background from membrane to membrane.
The automated gel staining capabilities of Processor Plus include nine preprogrammed and four user-defined protocols for proteins and nucleic acids. A choice of small- and large-format tray sizes accommodates one to six mini-gels or one to two standard size gels for silver- or Coomassie blue-staining.
The components behind the intelligent fluid handling of Processor Plus include a high-speed peristaltic pump for fast delivery and removal of reagents; a 10-port valve for the creation of multiple reagent protocols and segregation of hazardous waste; and a rhomboid-shaped tray with smooth and even rocking for rapid fluid mixing and coverage.
Protocol entry and editing is simple with up to 30 steps allowed per protocol. A removable protocol key, which stores one protocol, protects critical protocols from accidental alteration.
A serial printer can be connected to the Processor Plus for report generation. The report provides information on each step including the volume of reagents used, the time (in min) for each step, and a validation report form, with spaces for sample ID, date, and operator.
|Complete Packsize||1 Kit|
1 x Tray support
2 x Blot processing mini tray
1 x Blot processing standard tray
1 x Blot lid assembly with valves and tubing
1 x Blot tray base
4 x Reagent bottle and a rack
2 x Wast bottle cap
1 x Spirit level
1 x Lid support
Get a jump ahead on your western blots and gels with the new Processor Plus
The new Hoefer Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both applications. Changing from one task to the other is as simple as switching trays. Gel staining or Western blot processing it’s your choice. Load the reagents,select a programmed protocol, and push "Start", the Processor Plus will fill and empty the tray with the right solution at the right time. Now you’re free to jump on more challenging and productive tasks. Close
2-D Electrophoresis, Principles and Methods
2-D electrophoresis is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique separates proteins according to two independent properties in two discrete steps. This handbook is intended to provide guidelines for performing high-resolution two-dimensional (2-D) electrophoresis. Techniques such as 2-D DIGE and different sample preparation methods are also included. Close
Automated staining of polyacrylamide gels with Hoefer Processor Plus
The new Processor Plus from Amersham Biosciences will help you get the jump on both Western blot processing and acrylamide gel staining. One intelligent fluid delivery system handles both applications. Changing from one task to the other is as simple as switching trays. Gel staining or Western blot processing it’s your choice. Load the reagents,select a programmed protocol, and push "Start", the Processor Plus will fill and empty the tray with the right solution at the right time. Now you’re free to jump on more challenging and productive tasks. 80-6343-34 Close
Convenient, reproducible and automated blot processing and gel staining using Processor Plus (from Life Science News, issue 3)
Hoefer™ Processor Plus™ automates membrane processing and gel staining following polyacrylamide gel electrophoresis. Ten protocols are pre-programmed for use with Western detection and gel staining kits from Amersham Biosciences. Users can also programme their own protocols or modify the standard protocols. Protein blot processing and silver staining with this instrument is convenient, reproducible, and sensitive. Close
Immunoblotting is a widely-used and powerful technique for the detection and identification of proteins using antibodies. The process involves the separation of sample proteins by polyacrylamide gel electrophoresis (PAGE) followed by the transfer of the separated proteins from the gel onto a thin support membrane. The membrane binds and immobilizes the proteins in the same pattern as in the original gel. The membrane (or “blot”) is then exposed to a solution containing antibodies that recognize and bind to the specific protein of interest. The antibodies bound to the membrane are detected by any of a variety of techniques, usually involving treatment with a secondary antibody. Although not difficult, the processing of the membrane is very time-consuming, the result of numerous successive incubations with a series of reagents and washes. The Processor Plus simplifies the processing of membranes by automating all of the solution changes and incubation steps once the blot has been generated. The processor delivers specified volumes of solution to the membrane surface, gently agitating the solutions evenly across the membrane surface for a set time, and then removes the solution. The precise timing and control of all of the steps results in increased reproducibility of immunoblots. Close
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For accessories not displayed in this section, please contact your local GE Healthcare sales representative.
|#||Product Name||Product Code||Price||Qty|
|1||Blot Processing Mini Tray||80-6444-42||No price available.||
|2||Blot Processing Standard Tray||80-6444-61||No price available.||
|3||Blot lid assembly||80-6454-68||No price available.||
|4||Blot processing tray base||80-6454-49||No price available.||
|5||Blot processing lid support||80-6455-25||No price available.||
|Pump tubing||80-6448-03||No price available.||
|Blotting tubing kit||80-6448-22||No price available.||
|Reagent bottles||80-6448-79||No price available.||
|Reagent labels||80-6455-06||No price available.||
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