Columns for analysis and purification of protein complexes

Superose 6 Increase is suitable for both preparative and analytical purposes.

These SEC columns are useful for the characterization of large proteins, protein complexes and the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.

Three SEC column sizes

Three size exclusion chromatography (SEC) column sizes are designed to serve different purposes:

• 10/300 GL column, 10 mm × 300 mm, for high resolution analysis as well as for small-scale (25–500 µL) preparative purification, such as the polishing step in a purification protocol
• 5/150 column, 5 mm × 150 mm, for rapid purity check and screening of small volumes (4–50 µL)
• 3.2/300 column, 3.2 mm × 300 mm, for high resolution analysis and purification of sample volumes 4–50 µl, when sample amount is limited and low buffer consumption is important

Feedback on Superose 6 Increase SEC columns

"Now I can work on more samples, resulting in higher throughput. I can now purify a protein in 30 minutes instead of an hour." Dr. Tomohiro Nishizawa (Nureki Laboratory). Department of Biological Sciences, Graduate School of Science, The University of Tokyo

Successful use in published research

Researchers use these prepacked columns for a variety of applications, such as:

• Study of a magnetic sensing protein
• Evaluation of effects two proteins have on microtubule nucleation
• Production of virus-like particles in E. coli